The utility model discloses an unclamping oil cylinder with an anti-loose structure, which comprises a supporting seat, the middle end of the supporting seat is fixedly connected with a fixing sleeve, the outer surface of the fixing sleeve is movably connected with a turntable, the surface of the turntable is provided with a second through groove, the periphery of the turntable is provided with first through grooves, and the first through grooves are communicated with the second through groove. The surface of the first through groove is movably connected with a vertical rod, the top of the vertical rod is fixedly connected with a baffle, the right side of the supporting seat is movably connected with a screw rod through a bearing, and the surface of the screw rod is in threaded connection with a moving plate. Through the arrangement of the positioning hole and the positioning block, the purpose of positioning the unclamping oil cylinder body and the supporting seat is achieved, horizontal displacement of the unclamping oil cylinder body is avoided, through the arrangement of the baffle, the unclamping oil cylinder body can be effectively limited in the vertical direction, displacement of the unclamping oil cylinder body in the vertical direction is avoided, and the unclamping oil cylinder body is prevented from moving in the vertical direction. Therefore, the unclamping oil cylinder body is effectively prevented from loosening.