The invention relates to sampling equipment with a good sealing effect, the sampling equipment comprises a fixed box, a moving plate, a sealing mechanism and a limiting mechanism, the sealing mechanism comprises a first spring, a tension meter, a sealing plate, an ejector pin and an adjusting assembly, and the limiting mechanism comprises a push rod, a sliding rod, a floating ball and a moving assembly. According to the sampling equipment with the good sealing effect, the fixed box is sealed through the sealing mechanism, and the restoring force of the first spring is controlled by adjusting the distance of the movable plate, so that sampling is performed on the fixed box in water areas with different depths, the use practicability is improved, and a floating ball is moved through the buoyancy of water. At the moment, the buoyancy of the floating ball is greater than the restoring force of the first spring and the pressure of water, so that the fixed box can be sealed, the volume of the main body in the fixed box can be controlled, and meanwhile, the main body at other depths is prevented from entering the fixed box in the moving-out process to influence the detection effect, and the detection accuracy is improved.