The invention provides a manufacturing method of a bullet-proof and puncture-proof material. The method comprises the following steps that a) high-strength fiber is woven into fabric, wherein the strength of the high-strength fiber is greater than or equal to 20 cN/dtex, and the tensile modulus is greater than or equal to 700 cN/dtex; b) the fabric obtained in the step a) is subjected to surface treatment, gum dipping, drying and winding in sequence, and fabric prepreg is obtained, wherein gum used in the gum dipping process is a thermoplastic resin matrix; and c) the fabric prepreg obtained in the step b) is subjected to hot press setting, and after cooling, the bullet-proof and puncture-proof material is obtained. Compared with the prior art, the manufacturing method provided by the invention takes the high-strength fiber as the raw material, the specific process steps and conditions are adopted, a good interaction effect is achieved, and the obtained bullet-proof and puncture-proofmaterial has both the excellent bullet-proof performance and puncture-proof performance, and is light in weight and comfortable to wear. Experiment results show that the surface density of the bullet-proof and puncture-proof material provided by the invention is below 240 g/m<2>, in addition, bullet-proof and puncture-proof standard requirements are met at the same time, and the material can be applied as the base material of bullet-proof and puncture-proof products.