A puncture-proof rain boot comprises a sole, a vamp and a shoe tube, the vamp and the shoe tube are integrally molded, the vamp and the shoe tube respectively have a three-layer structure and respectively concretely comprise a waterproof layer, a buffering and puncture proofing layer and a lining 
foot protection layer from outside to inside, above three 
layers are sequentially bonded together, the waterproof layer is made of rubber or a PVC material, and the buffering and puncture proofing layer is made of a puncture-proof high 
toughness material and prevents pointed hard objects from 
puncturing the vamp; the buffering and puncture proofing layer is made from, by weight, 30-60 parts of nylon6, 20-40 parts of short glass fibers, 1-1.2 parts of dimethicone, 0.5-1 part of an 
antioxidant, 5-10 parts of linear 
polyethylene, 3-5 parts of 
polylactic acid, 1-5 parts of a synergistic agent, 0.5-1 part of a nucleating agent, 0.5-1 part of an 
ultraviolet ray absorber, 0.5-1 part of a surfactant, 1-3 parts of a chain 
extender, 0.5-1 part of dry oxhide 
gelatin powder and 0.5-1 part of ceramsite nanopowder; and the lining 
foot protection layer is formed by blending 
polyester fibers, bamboo fibers and man-made spider fibers, and the lining 
foot protection layer has good elasticity and 
toughness, allows skins in contact with the lining foot 
protection layer to be comfortable, and has an 
antibacterial effect.