The invention discloses a negative oxygen ion generator. The negative oxygen ion generator comprises a shell, an air inlet filtering assembly, a fan pressurization assembly, a water spraying cavity, an air outlet cavity and a water supplementing tank, wherein the air inlet filtering assembly comprises a filtering layer, a chemical treatment layer and a drying layer, the fan pressurization assembly comprises an air exhaust impeller and a vortex cavity, the bottom of the water spraying cavity is filled with water, and labyrinth filtering layers are arranged on the two sides of the water spraying cavity; an aerator is clamped between the labyrinth filtering layers and comprises a driving water wheel blade, an impact cavity and a driven water wheel blade; and a water adding port is arranged at the upper end of the water supplementing tank. According to the negative oxygen ion generator, a motor rotates to drive the air exhaust impeller to suck outdoor air into the tank body, the outdoor air passes through the air inlet filtering assembly after being primarily filtered, after being pressurized by the vortex cavity and rotating at a high speed, the water is driven by the water wheel blades to impact the inner wall of an oxygenation cavity to form high-concentration negative oxygen ions, and the high-concentration negative oxygen ions are blown out from air outlets after being combined with the air, so that the negative oxygen ion generator is suitable for being used in environments such as families, office buildings, shopping malls and ecological oxygen bars with larger spaces, and is low in manufacturing cost.