39 results about "High pressure freezing" patented technology

The invention discloses a dairy drink of fresh sea cucumber (sea cucumber polypeptide, sea cucumber amylase) processed by a low-temperature extra high pressure freezing out micro mist technology and a preparing method, including 1, low temperature extra high pressure sterilizing: after being washed, the sea cucumber is put into a closed container and kept for 15 minutes within 20 DEG C under the pressure of 500-1000MPa and the autolytic enzyme and bacilli of the sea cucumber are blanched; 2, freezing out: after being sterilized, the sea cucumber is quickly frozen to be ice at the temperature below 5 DEG C below zero within 0.5 hour and leading the ice to sublime (15-20 DEG C) as vapor to be drained out under the vacuum condition (40-50mm mercury column) to lead the water content of the sea cucumber to be lower than 3 percent; 3, micro mist processing: after being frozen out, the sea cucumber is crushed roughly and then a fluid-bed fluid jet mill is adopted to lead the diameter of the micro mist of the sea cucumber to be between 3 to 5 Mum; 4, a physical sterilization method contains no chemical additives, thus keeping the biological activities of various matters like the sea cucumber polypeptide and the sea cucumber amylase in the sea cucumber and the invention combined with the balanced nutrition components of the dairy drink, thus enhancing the health effects of the sea cucumber of delaying senility and improving immunity.

The invention discloses a method for prolonging the storage period of litchis. Vacuum packed fresh litchis are subjected to high pressure freezing, and are then put in storage and frozen. The high pressure freezing specifically includes: under a high pressure condition of 200-400MPa, lowering the temperature to a range from -18DEG C to -20DEG C, and then releasing the pressure to atmospheric pressure within 2-5s. According to the method for prolonging the storage period of litchis involved in the invention, after one-year freezing, the unfrozen litchis have almost the same quality to that of fresh fruits, small juice leakage, and no obvious change on the skin color.

The invention discloses a milk of fresh sea cucumber (sea cucumber polypeptide, sea cucumber amylase) processed by a low-temperature extra high pressure freezing out micro mist technology and a preparing method, including 1, low temperature extra high pressure sterilizing: after being washed, the sea cucumber is put into a closed container and kept 15 minutes within the temperature of 20 DEG C under the pressure of 500-1000MPa and the autolytic enzyme and bacilli of the sea cucumber are blanched; 2, freezing out technology: after being sterilized by superhigh pressure, the sea cucumber is quickly frozen to be ice under at the temperature below 5 DEG C below zero within 0.5 hour and leading the ice to sublime(15-20 DEG C) as vapor to be drained out under the vacuum condition (40-50mm mercury column) to lead the water content of the sea cucumber to be lower than 3 percent; 3, micro mist processing: after being frozen out, the sea cucumber is crushed roughly and then a fluid-bed fluid jet mill is adopted to lead the diameter of the micro mist of the sea cucumber to be between 3 to 5 Mum; 4, the invention adopts a physical processing and contains no chemical additives, thus keeping the biological activities of various matters like the sea cucumber polypeptide and the sea cucumber amylase in the sea cucumber; and simultaneously the invention is combined with the balanced nutrition components of the fresh milk, thus having the advantages of full nutrition, delaying senility and improving immunity.

The invention discloses a pressure-adjustable mine high- and low-pressure water energy exchange system. The pressure-adjustable mine high- and low-pressure water energy exchange system comprises an underground integrated control center system of a monitoring system, which comprises an industrial control computer and is connected with a hydraulic station and a three-cavity water energy exchange device. While high-pressure freezing water is subjected to pressure relief, hot water is conveyed to the ground surface from the underground part through a water energy conversion pipe, so that the potential energy exchange between the cold water and the hot water is realized; the opening degree of an electric flow adjusting valve is controlled through signals of the temperature and the flow acquired by a temperature sensor and a flow sensor, and the flow of the cold water is adjusted to be matched with end load; an underground circulating water treatment unit guarantees the backwater quality of the hot water, makes sure the quality of the water flowing through the valve, and prolongs the service life of the valve; a variable frequency water complementing system guarantees the system to stably run; and the hydraulic station is used for controlling the valve to open and close according to a certain logic sequence through an instruction of the industrial control computer, guaranteeing the system to continuously and stably run, and monitoring the status of the system equipment.

Methods of sterilizing a temperature sensitive material, such as an acidified or non-acidified food product, pharmaceutical product or cosmetic product, are disclosed. The methods comprise freezing the temperature sensitive material to an initial temperature of less than or equal to −2° C. and then either (i) pressurizing the frozen temperature sensitive material to a first elevated pressure of at least 250 MPa for a predetermined first period of time of at least 3 minutes or (ii) pressurizing the frozen temperature sensitive material to a first elevated pressure of at least 250 MPa for a predetermined first period of time of at least 90 seconds, releasing the first elevated pressure for a predetermined pause period of time and then pressurizing the temperature sensitive material to a second elevated pressure of at least 250 MPa for a predetermined second period of time of at least 90 seconds.

An automatic loading apparatus (12) for specimens (1) for high-pressure cryosubstitution is disclosed. The automatic loading apparatus (12) is connected to a high-pressure freezing device (40). Specimens (1) are inserted in a holder (2). By means of a slider (4), the specimens (1) and holders (2) are conveyed to the automatic loading apparatus (12). The guidance element (26) allows exact positioning of the holder (2) in the clamping element (14). The automatic loading apparatus makes possible rapid transfer of the holder (2) having the specimen (1) from, for example, an optical microscope to the high-pressure freezing device (40).

The present invention relates to modular specimen holder (10') for high pressure freezing and / or X-ray crystallography of a specimen comprising a specimen holding element (100') and an extension element (200') connectable with each other and separable from each other; the specimen holding element (100') comprising a tubule (120') and a base element (110'), wherein the tubule (120') is adapted to hold the specimen, the base element (110') is adapted to hold the tubule (120'), wherein a distance from a bottom of the base element (110') to a top of the tubule (120') is a distance d1; the extension element (200') being adapted to be connected with the base element (110'), wherein, when the extension element (200'} and the base element (110') are connected with each other, a distance from a bottom of the extension element (200') to the top of the tubule (120') is a second distance d2; wherein the second distance d2 is larger than the first distance d1.

The present invention relates to modular specimen holder (10′) for high pressure freezing and / or X-ray crystallography of a specimen comprising a specimen holding element (100′) and an extension element (200′) connectable with each other and separable from each other; the specimen holding element (100′) comprising a tubule (120′) and a base element (110′), wherein the tubule (120′) is adapted to hold the specimen, the base element (110′) is adapted to hold the tubule (120′), wherein a distance from a bottom of the base element (110′) to a top of the tubule (120′) is a distance d1; the extension element (200′) being adapted to be connected with the base element (110′), wherein, when the extension element (200′) and the base element (110′) are connected with each other, a distance from a bottom of the extension element (200′) to the top of the tubule (120′) is a second distance d2; wherein the second distance d2 is larger than the first distance d1.

The invention relates to a pretreatment method for smelting and extracting harmful substances in a milk sample in a freezing region, and in particular relates to a pretreatment method for smelting and extracting harmful substances in the milk sample in the freezing region. The pretreatment method is mainly used for measuring residues of choramphenicols in infant formula milk powder, and belongs to the field of food processing and detection. The method comprises the following steps: (1) weighing a solid milk powder sample and adding ultra-pure water or weighing the milk powder sample, mixing the milk powder sample with an organic extraction reagent, and performing extraction and oscillation in sequence to obtain treatment liquid; (2) pouring the treatment liquid into a high-pressure freezing extraction kettle, and injecting ethyl acetate; (3) putting the high-pressure freezing extraction kettle into a refrigerator for freezing or into an ice bath; and (4) taking out the high-pressure freezing extraction kettle, sucking an organic phase on the upper layer, and filtering to obtain filtrate. The pretreatment method for smelting and extracting the harmful substances in the milk sample in the freezing region is high in extraction efficiency; interference components can be reduced and even eliminated; furthermore, the method is easy and convenient to operate, and effective detection on the harmful substances in the milk sample is facilitated.

An automatic loading apparatus (12) for specimens (1) for high-pressure cryosubstitution is disclosed. The automatic loading apparatus (12) is connected to a high-pressure freezing device (40). Specimens (1) are inserted in a holder (2). By means of a slider (4), the specimens (1) and holders (2) are conveyed to the automatic loading apparatus (12). The guidance element (26) allows exact positioning of the holder (2) in the clamping element (14). The automatic loading apparatus makes possible rapid transfer of the holder (2) having the specimen (1) from, for example, an optical microscope to the high-pressure freezing device (40).

The invention discloses a gas throttle type cryosurgical device and a method for controlling the same. A plurality of rewarming processes can be implemented by the aid of the gas throttle type cryosurgical device and the method. Rewarming (shunt rewarming) effects can be realized by the aid of gas which is shunted from high-pressure freezing gas and has limited pressures lower than throttle working pressures; rewarming (residual gas rewarming) effects can be realized by the aid of residual gas with the pressures lower than freezing working pressures; rewarming (normal-pressure rewarming) effects can be realized by the aid of normal-pressure gas; rewarming (throttle rewarming) effects can be realized by the aid of throttle pyrogenic gas; rewarming (exhaust rewarming) effects can be realized by the aid of gas source shutting and equipment exhaust procedures. The gas throttle type cryosurgical device and the method have the advantages that the gas throttle type cryosurgical device is also provided with gas preheating devices, accordingly, the rewarming efficiency can be improved, the rewarming temperatures can be increased, and the gas throttle type cryosurgical device has a freezing function, a rewarming function and a heating function; the technical prejudice that throttle frigorific gas only can be used for freezing can be corrected, rewarming difficult problems of existing gas throttle type cryosurgical equipment can be solved, the gas utilization rate can be increased, the surgical cost can be reduced, energy can be saved, environments can be protected, the rewarming performance can be enhanced, the gas throttle type cryosurgical device has an additional thermotherapy function, and the gas throttle type cryosurgical device and the method are beneficial to promoting popularization and improvement of cryoablation technologies.

The invention belongs to the technical field of food and mainly relates to a method of utilizing soybean aqueous enzymatic method hydrolysate and residue to prepare Qianye tofu. The method includes following steps: adopting an aqueous enzymatic method to extract soybean to obtain free oil, emulsion, hydrolysate and residue; spray-drying the hydrolysate to obtain aqueous enzymatic method soybean protein; homogenizing ice water, the aqueous enzymatic method soybean protein and the aqueous enzymatic method residue, adding soybean oil, glutamine transaminase, tapioca modified starch, edible salt, gourmet powder and white sugar, mixing, and pulping to obtain paste; scraping the surface of the paste to be flat; putting in a disc, freezing at high pressure to obtain a formed product, dicing, and freeze-storing at normal pressure to obtain Qianye tofu. High-pressure freezing technology is utilized, so that compared with Qianye tofu produced by conventional processing modes, ice crystals formed by freezing are small in size and uniform in distribution in the Qianye tofu, water loss is small, and quality and taste of the Qianye tofu are improved effectively.

The invention discloses a preparation method of frozen ultrathin slices. The method comprises the following steps: cutting a tissue sample by using a vibration slicer to obtain thin slices with the thickness of not more than 200 mu m; putting the thin slices into a sample table for high-pressure freezing; transferring the sample table loaded with the thin slices to a frozen ultrathin slicing machine, and pre-thinning the sample table loaded with the thin slices; transferring the pre-thinned sample table into a scanning transmission electron microscope; preparing a frozen ultrathin slice by using the frozen focused ion beam. Therefore, the thickness of the frozen ultrathin section prepared by the method is about 200nm and does not exceed 300nm, and the requirement of scanning transmission electron microscope imaging can be well met.

The invention discloses an acid permeation high-pressure freezing wall breaking technology for Ganoderma lucidum spore. Through the scheme, Ganoderma lucidum spore is firstly permeated and soaked withacid, and then through high pressure wall breaking and freezing wall breaking, the Ganoderma lucidum spore can be completely broken, and the content of the Ganoderma lucidum spore is completely released. Specifically, the Ganoderma lucidum spore without wall breaking is permeated and soaked with acid, sterilization high pressure wall breaking is carried out, the Ganoderma lucidum spore is dried inside an oven at the temperature of 65 DEG C, then is frozen inside a refrigerator at the temperature of 4 DEG C, and then is dried inside an oven at the temperature of 65 DEG C so as to complete thewall braking function of the Ganoderma lucidum spore. The wall braking rate of the high pressure freezing wall breaking method can reach more than 99%, wherein the content of the main effective ingredient ganoderan of the Ganoderma lucidum spore is 2.5 to 3.5 times of that before wall breaking, and is 1.5 to 2.0 times of the content of the wall-broken Ganoderma lucidum spore in a traditional wallbreaking technology. Moreover, the wall breaking technology accords with the sanitary condition, the content of the Ganoderma lucidum spore is not exposed, the oxidative deterioration of the content is unlikely to cause, the product can be directly filled into capsules or crushed into tablets, and the Ganoderma lucidum spore also can be stored for a long time in the condition without contamination.

Methods of sterilizing a temperature sensitive material, such as an acidified or non-acidified food product, pharmaceutical product or cosmetic product, are disclosed. The methods comprise freezing the temperature sensitive material to an initial temperature of less than or equal to −2° C. and then either (i) pressurizing the frozen temperature sensitive material to a first elevated pressure of at least 250 MPa for a predetermined first period of time of at least 3 minutes or (ii) pressurizing the frozen temperature sensitive material to a first elevated pressure of at least 250 MPa for a predetermined first period of time of at least 90 seconds, releasing the first elevated pressure for a predetermined pause period of time and then pressurizing the temperature sensitive material to a second elevated pressure of at least 250 MPa for a predetermined second period of time of at least 90 seconds.

The invention relates to a cooling device for spraying cold water into a lobster culture trench applying chilled water made by solar energy, belonging to the technical field of new energy refrigeration. Lithium bromide solution is used as an absorbent and water is used as a refrigerant for a solar-driven lithium bromide-water absorptive refrigerator; solar energy is used as a driving heat source; the driving heat source is directly or indirectly utilized in a unit for making chilled water and discharging cold air. The chilled water output from a chilled water tank is input into a booster pump for boosting through a chilled water delivery pipe; the boosted high-pressure chilled water is uniformly sprayed into the lobster culture trench through a high-pressure chilled water delivery pipe and a chilled water spray gun head, so that the water temperature in the lobster culture trench is reduced in the high-temperature season of summer, and the most proper growth temperature of lobsters is kept at 25-30 DEG C. The food intake of the lobsters is increased, and the growth speed of the lobsters is increased. Sunlight irradiates a solar cell to generate current; the current is input into a controller through a wire; the current which is output from the controller is used for providing power for the energy storage battery and the booster pump through wires.

The present invention discloses a cryosurgery device capable of achieving freezing and rewarming by adjusting gas pressure. The cryosurgery device comprises a high-pressure gas source, a high-pressure pressure-limiting valve, a freezing gas main pipe and a freezer for throttling and freezing; high-pressure gas divided from a high-pressure gas pipe passes through a shunting electromagnetic valve and a low-pressure pressure-limiting valve for pressure limiting, and is transmitted to the freezer via a heating gas main pipe for shunting and rewarming; gas from a low-pressure gas source passes through a low-pressure electromagnetic valve and the low-pressure pressure-limiting valve for pressure limiting, and is transmitted to the freezer via the heating gas main pipe for low-pressure rewarming; and the heating gas main pipe is provided with a gas heater capable of preheating low-pressure rewarming gas and achieving a controllable heating function. The technical prejudice that throttling refrigeration type gas can only be used for freezing is corrected, warming is performed by utilization of residual gas of high-pressure freezing gas and low-pressure gas, the rewarming problem and low-temperature working medium waste problem of a gas throttling type cryosurgery device are solved, and freezing, rewarming and heating functions are achieved.

The invention relates to a device (10) for preparing a microscope sample (1) for a high-pressure freezing process, in which the sample (1) is provided using an arrangement of an intermediate plate (33) comprising a high-pressure freezing cartridge (30) and a culture chamber (100, 200), the intermediate plate (33) being attached to the culture chamber (100, 200) and being detachable from the culture chamber (100, 200) by effecting a relative movement between the intermediate plate (33) and the culture chamber (100, 200), the culture chamber (100, 200) is provided with an opening (36), and the sample (1) is provided on an encapsulation element (37) to be fitted into the opening (36) of the intermediate plate (33), in which the device (10) comprises an engagement structure (11, 21) adapted to engage with the intermediate plate (33) and restrict movement of the intermediate plate (33) when the culture chamber (100, 200) is moved in order to effect said relative movement between the intermediate plate (33) and the culture chamber (100, 200), thereby detaching the intermediate plate (33) from the culture chamber (100, 200). Furthermore, a culture chamber (100, 200), an intermediate plate (33) of a high-pressure freezing box (30), an arrangement comprising these components and a corresponding method are also part of the invention.

The invention discloses a heat exchange system for ultrahigh-pressure refrigeration equipment. The heat exchange system includes an external heat exchange assembly which is arranged outside a high-pressure freezing cavity of the ultrahigh-pressure freezing equipment, and an internal heat exchange assembly which is arranged in the high-pressure freezing cavity. The built-in heat exchange assembly is spirally arranged on the inner wall of the high-pressure freezing cavity; a refrigerant inlet and a refrigerant outlet of the built-in heat exchange assembly are both formed in a bottom plug at thebottom of the high-pressure freezing cavity and connected with an external water pump; and thermal power for promoting a medium to flow is established in the high-pressure freezing cavity. By arranging the built-in heat exchange assembly in a high-pressure freezing cavity of the ultrahigh-pressure freezing equipment., the heat exchange pipe of the built-in heat exchange assembly is spirally arranged along the axis of the inner wall of the high-pressure freezing cavity and a medium placed in the high-pressure freezing cavity can be directly cooled, wherein the medium on the upper portion in thehigh-pressure freezing cavity and the medium on the lower portion in the high-pressure freezing cavity form a circularly-flowing thermal power field, so that on one hand, heat exchange is enhanced through convection, on the other hand, the temperature of the thermal power field is uniform, and heat balance is achieved.

The invention discloses a pressure-adjustable mine high- and low-pressure water energy exchange system. The pressure-adjustable mine high- and low-pressure water energy exchange system comprises an underground integrated control center system of a monitoring system, which comprises an industrial control computer and is connected with a hydraulic station and a three-cavity water energy exchange device. While high-pressure freezing water is subjected to pressure relief, hot water is conveyed to the ground surface from the underground part through a water energy conversion pipe, so that the potential energy exchange between the cold water and the hot water is realized; the opening degree of an electric flow adjusting valve is controlled through signals of the temperature and the flow acquired by a temperature sensor and a flow sensor, and the flow of the cold water is adjusted to be matched with end load; an underground circulating water treatment unit guarantees the backwater quality of the hot water, makes sure the quality of the water flowing through the valve, and prolongs the service life of the valve; a variable frequency water complementing system guarantees the system to stably run; and the hydraulic station is used for controlling the valve to open and close according to a certain logic sequence through an instruction of the industrial control computer, guaranteeing the system to continuously and stably run, and monitoring the status of the system equipment.

The present invention relates to a pretreatment method for harmful substances in milk powder samples, in particular to a pretreatment method for smelting and extracting harmful substances in milk samples in a frozen region, mainly used for chloramphenicol residues in formula milk powder for infants and young children The method for measuring belongs to the field of food processing detection. The method comprises the following steps: (1) taking a solid milk powder sample, adding ultrapure water, or weighing an emulsion sample, mixing it with an organic extraction reagent, first extracting, then shaking to obtain a treatment solution; (2) pouring the treatment solution (3) Put the high-pressure freezing extraction kettle into the refrigerator to freeze, or put it in an ice bath; (4) Take out the high-pressure freezing extraction kettle, absorb the upper organic phase, and filter to obtain the filtrate. The pretreatment method for extracting harmful substances in milk samples by smelting in a frozen region provided by the present invention not only has high extraction efficiency, can reduce or even eliminate interfering components, but also is simple and convenient to operate, and is helpful for effective detection of harmful substances in milk samples.

The invention discloses a cleaning system for volatile solvent recycling. A seal cavity is included and internally comprises a rough cleaning groove, a fine cleaning groove, a drying condenser, a distillation condenser, a buffering groove, a distillation still and a circulation pump. The lower portions of the rough cleaning groove and the fine cleaning groove are connected to the circulation pumpthrough a filter. One side of the rough cleaning groove is connected to the distillation still, and one side of the fine cleaning groove is connected to the buffering groove. The distillation still isconnected to the drying condenser and the distillation condenser. The circulation pump is in pipeline connection to the drying condenser. The cleaning system is simple in structure, reasonable in design and convenient to use, since a whole volatile solvent circulates in the seal cavity, component cleaning and volatile solvent recycling are achieved through seal door sealing, classified solvent injection, high-pressure freezing drying and multi-direction scavenging, the usage amount of the solvent is reduced, cost is saved, and health and safety of operation personnel are guaranteed.

The invention discloses a gas throttle type cryosurgical device and a method for controlling the same. A plurality of rewarming processes can be implemented by the aid of the gas throttle type cryosurgical device and the method. Rewarming (shunt rewarming) effects can be realized by the aid of gas which is shunted from high-pressure freezing gas and has limited pressures lower than throttle working pressures; rewarming (residual gas rewarming) effects can be realized by the aid of residual gas with the pressures lower than freezing working pressures; rewarming (normal-pressure rewarming) effects can be realized by the aid of normal-pressure gas; rewarming (throttle rewarming) effects can be realized by the aid of throttle pyrogenic gas; rewarming (exhaust rewarming) effects can be realized by the aid of gas source shutting and equipment exhaust procedures. The gas throttle type cryosurgical device and the method have the advantages that the gas throttle type cryosurgical device is also provided with gas preheating devices, accordingly, the rewarming efficiency can be improved, the rewarming temperatures can be increased, and the gas throttle type cryosurgical device has a freezing function, a rewarming function and a heating function; the technical prejudice that throttle frigorific gas only can be used for freezing can be corrected, rewarming difficult problems of existing gas throttle type cryosurgical equipment can be solved, the gas utilization rate can be increased, the surgical cost can be reduced, energy can be saved, environments can be protected, the rewarming performance can be enhanced, the gas throttle type cryosurgical device has an additional thermotherapy function, and the gas throttle type cryosurgical device and the method are beneficial to promoting popularization and improvement of cryoablation technologies.