7 results about "High pressure freezing" patented technology

A method of sample preparation in association with an electron microscope. In one embodiment, a carrier supporting a biological sample is received. A high-pressure freezing (HPF) protocol is then applied to the carrier. The protocol comprises a first phase, and a second phase that follows the first phase. The first phase comprising (i) comprising: a pressurizing operation applying a pressure to the carrier, and (ii) a rapid cooling operation using a coolant that avoids a Leidenfrost effect at a carrier-coolant interface by maintaining nucleate boiling or substantially suppressing film boiling upon contact with the carrier. The pressurizing and rapid cooling operations freeze the biological sample while preserving its structural integrity. The second phase involves raising a temperature of the carrier and the frozen biological sample to a given temperature for one of: storage, and use.

The invention discloses a mine cooling chilled water high-low pressure conversion device which comprises a high-pressure chilled water pressure reduction module and a low-pressure chilled water pressurization module. High-pressure chilled water enters the water tank through the water inlet pipe, pressure relief is conducted through volume buffering of the water tank, then the hydraulic fins are impacted to drive the main shaft and the main gear to rotate, secondary pressure relief is achieved, and low-pressure chilled water is output. The main gear drives the transmission gear to convert rotary motion into reciprocating motion of the sliding rod, a piston in the pressure cylinder is driven to pressurize return water, and check valves are arranged on a liquid inlet branch and a liquid outlet branch to guarantee one-way flow. The device can be arranged in multiple groups in parallel or in series, key parts are made of wear-resistant steel, electrical elements are not included, mechanical transmission is achieved through hydraulic potential energy of high-pressure chilled water, the structure is reliable, the device is suitable for the underground high-humidity high-pressure low-temperature environment, the anti-explosion requirement and the operation risk are lowered, pipe network pressure fluctuation is reduced, and the system energy efficiency is improved.

The present application belongs to the technical field of transmission electron microscope sample preparation, and provides a preparation method of a hydrogel material transmission electron microscope sample. The preparation method comprises: fixing the structure of the hydrogel material by high-pressure freezing, freezing sectioning the hydrogel material after the structure is fixed to obtain a hydrogel ultrathin section; pulling the hydrogel ultrathin section to a support grid to make the hydrogel ultrathin section adhere to the support grid; placing the support grid in liquid nitrogen for storage, and then sending the support grid into a cryogenic transmission electron microscope through a cryogenic transmission system for microstructure characterization. The present application fixes the structure of the hydrogel by high-pressure freezing to retain the original structure of the hydrogel; the ultrathin sectioning is performed under the condition of freezing, and the hydrogel does not need to be embedded in resin; the sectioning is directly performed under the condition of low-temperature freezing and the section is adhered to the support grid, the support grid is sent into the cryogenic transmission electron microscope through the cryogenic transmission system, and the structure characterization is performed under a low electron dose, so that the accuracy of the microstructure of the hydrogel material and the distribution information of the nanofiller is improved.

According to the preservation method for the glycocalyx ultrastructures of podocytes, endothelial cells and epithelial cells, a high-pressure freezing instrument and a freezing replacement instrument are adopted, millisecond-level glassy state fixation of a sample is achieved through physical freezing, low-temperature dehydration is combined, and the dual problems of chemical damage and charge interference are effectively avoided; the shape preservation effect of glycocalyx of glomerular podocytes, glomerular endothelial cells and small intestine epithelial cells is remarkably improved, the thickness and spatial arrangement of the glycocalyx are close to the physiological state, and dangerous chemicals are prevented from being used.

The application belongs to the field of food processing, and particularly relates to a method for improving the freezing characteristics of soybean protein isolate plant protein meat. Soybean 7S globulin and soybean 11S globulin are mixed as soybean protein isolate at a 7S / 11S mass ratio of 0.8-1.2:1, other plant proteins and auxiliary materials including TG enzyme high-temperature-resistant microcapsules are compounded, and a high-moisture extrusion process is used for preparation; after forming, heat setting, gradient cooling pretreatment and ultra-high pressure freezing treatment are performed to obtain plant protein meat with good freezing characteristics. The soybean protein isolate plant protein meat prepared by the method has a water holding capacity of 3.5-4.5 g / g, a fiber integrity of 82-95%, an elastic modulus of 250-320 KPa, a freeze-thaw syneresis rate of 15-25%, a gel strength of 35-45 N, a cooking loss rate of 5-15%, and a weakly bound free water proportion of 78-97% after one freeze-thaw cycle.

The invention discloses an ultrahigh-pressure freezing preservation device and method, and relates to the technical field of freezing preservation, the ultrahigh-pressure freezing preservation device comprises an inner cylinder, the inner cylinder is sleeved with an outer cylinder, the upper end of the inner cylinder and the upper end of the outer cylinder are both fixedly connected with a feeding seat, the lower end of the inner cylinder and the lower end of the outer cylinder are both fixedly connected with a discharging seat, and a water inlet pipe is fixedly connected to the feeding seat; the inner cylinder is sleeved with a cooling coil, the cooling coil is located between the inner cylinder and the outer cylinder, and a pressure release valve is installed on the outer cylinder; water is injected into the inner cylinder and pressurized, then cooling is carried out through the cooling coil, under the protection of an ultrahigh pressure state, growth of ice crystals can be inhibited, water can form non-crystallization, water in material tissue cells rapidly forms fine ice crystals under pressure, and the fine ice crystals are disorderly arranged to form vitrified ice crystals; the cells of the frozen materials are quickly fixed, and cell walls are not punctured by ice crystals, so that the taste of the materials after unfreezing is guaranteed, and more nutritional ingredients are reserved.

A method of sample preparation in association with an electron microscope. In one embodiment, a carrier supporting a biological sample is received. A high-pressure freezing (H PF) protocol is then applied to the carrier. The protocol comprises a first phase, and a second phase that follows the first phase. The first phase comprising (i) comprising: a pressurizing operation applying a pressure to the carrier, and (ii) a rapid cooling operation using a coolant that avoids a Leidenfrost effect at a carrier-coolant interface by maintaining nucleate boiling or substantially suppressing film boiling upon contact with the carrier. The pressurizing and rapid cooling operations freeze the biological sample while preserving its structural integrity. The second phase involves raising a temperature of the carrier and the frozen biological sample to a given temperature for one of: storage, and use.