The invention discloses a UHPLC-MS/MS (Ultra High Performance Liquid Chromatography-Mass Spectrometry-Mass Spectrometry) analytical method for measuring rat curcumin plasma concentration. The method comprises the following steps: (1) preparing a curcumin standard solution and an internal standard tinidazole standard solution, and establishing a standard curve; and (2) adding an internal standard into to-be-measured plasma, performing pretreatment, performing LC-MS/MS analysis, and calculating the concentration of curcumin in the to-be-measured plasms by utilizing the standard curve. According to the method disclosed by the invention, the chromatographic conditions and internal standard substances are reasonably selected, the sensitivity is improved, the analysis time is shortened, and the sample pretreatment process is simplified. Meanwhile, the method disclosed by the invention has high accuracy and precision degree, and the detection requirements of non-clinical pharmacokinetic study are met.