The invention relates to the technical field of sine wave oscillator output voltage auxiliary circuits, and particularly discloses an RC sine wave oscillator output voltage regulation and control circuit which comprises a sampling circuit, a regulation and control circuit and a two-phase oscillation circuit, the sampling circuit is connected with the regulation and control circuit, and the regulation and control circuit is connected with the two-phase oscillation circuit. The regulating circuit is used in cooperation with a sine wave oscillator composed of an R operational amplifier, a C operational amplifier and the like, so that voltage generated by oscillation can be controlled by an external direct-current voltage, and the voltage can be continuously and accurately regulated and controlled between 0 and the maximum value. Therefore, the functions of voltage stabilization, voltage regulation, current stabilization, power stabilization and the like can be conveniently realized by matching with the amplifier. And the control of a computer can be conveniently accepted, a plurality of regulation and control functions are realized, and a plurality of application requirements are met.