The invention relates to a desktop and handheld dual-purpose mouse and a using method of the desktop and handheld dual-purpose mouse. The desktop and handheld dual-purpose mouse comprises a handheld track control device, an upper cover 2, a roller device 3, a desktop right key button 4, a desktop left key button 5, a handheld left key button 6, a signal transmission device, a circuit board 8, a lower cover 9, a handheld right key button 10 and a desktop track control device. The desktop track control device may be an optical sensor 11 or a track ball; the handheld track control device may be a track ball 1, a touch plate 12 or a point rod 14, and the signal transmission device may be a signal wire 7 or infrared and Bluetooth wireless transmission device 13. The desktop and handheld dual-purpose mouse is completely consistent with the existing mouse when being used as a desktop mouse, can casually and comfortably achieve information exchange with the computer when being handheld to use, accords with the existing operational habits in use, can effectively reduce fatigues of arms, prevents an occupational disease of a mouse hand and is wide in application prospect.