The present invention relates to the composition and method of preparing an
immunogen designated as I-spga consisting of a complex
antigen prepared from 18 to 26 species of pathogenic microorganisms isolated from patients, inactivated with binary ethyleneamine (BEI) and formalin, diluted in a SPGA
immunopotentiator mixed with QS-21
adjuvant. By inoculating the hens with the I-spga
immunogen, hyperimmune eggs (Immunospga) are obtained which contain immunologically active proteins specific to the 18-26 antigens used for immunization. The immune response of the hens is specific to the used antigens by amplification of the antigenic
signal by the SPGA
immunopotentiator and due to a special
immunization program that allows the
immune system to act complex and intense: The I-spga complex
antigen contains 18-26 microorganisms isolated from patients, bacterial bodies, components from bodies obtained by
ultrasonography, cilia, exotoxins, endotoxins, spores, viruses, fungi or yeasts. This pathogenic material is inactivated with BEI and formalin. The I-spga
antigen is of three types. The standard I-spga antigen is composed of 18 to 24 antibiotic-resistant bacterial species isolated from patients in Romania. The specific I-spga complex antigen is composed of the I-spga complex antigen containing a mixture of 7-9 strains from a
single species of
bacteria, fungi or yeasts isolated from patients in Romania mixed with SPGA and QS-21, used for
inoculation of hens previously immunized with standard I-spga antigen. The personalized I-spga antigen is composed of patient-derived
pathological material containing
cellular debris and pathogenic germs inactivated with BEI and formalin and mixed with SPGA and QS-21 and is used to immunize hens previously immunized with the standard I-spga antigen. This now patented technology encompasses a new generation of biological products in which the immune response of the hens to different groups of parenterally inoculated antigens at different time intervals is overlapping. Chicken response is uniform and additional administration of immunogens and SPGA as an
immunopotentiator amplifies the antigenic
signal and immune response. The I-spga
immunogen as well as the immune response contain two markers, G and A, which identify the I-spga antigen used for immunization against the antigens used to produce the Imunoinstant group bio-preparations or similar products. The I-spga immunogen is used to immunize the hens for obtaining immunologically active proteins that can be used to treat immune deficiencies,
psoriasis,
epidermolysis bullosa, other dermatitises, nosocomial infections, antibiotic-resistant infections in the
urinary system of children and grownups.