The invention relates to a virus culture medium transferring device. The virus culture medium transferring device comprises a box body, a virus storage tube, a culture medium reservoir, a detergent reservoir, a waste liquor reservoir, an automatic sample introduction device, a sensor system, an automatic control device, a pumping system and a sampler, wherein the sampler is located in an accommodating structure of the external wall of the box body, the virus storage tube is located inside the box body and is arranged around the automatic sample introduction device, and the automatic sample introduction device is connected with the culture medium reservoir, the detergent reservoir and the waste liquor reservoir through pipelines and is provided with the pumping system. According to the virus culture medium transferring device, automatic sampling, automatic collecting and no splashing during opening can be achieved, and thus, the virus culture medium transferring device has the characteristics of miniaturization, automation and integration.