The invention discloses an automatic inspection machine of a step electrode. The automatic inspection machine comprises a rack, an image collection device, a transverse drive device, an industrial personal computer and a display screen, wherein the rack is provided with a detection platform and comprises a microscope stage and a servo system; the image collection device is located at the upper part of the microscope stage and comprises a base frame, a Z-axis adjusting mechanism, a shooting mechanism and a code reading mechanism; the Z-axis adjusting mechanism, the shooting mechanism and the code reading mechanism are arranged on the base frame; the Z-axis adjusting mechanism comprises a PLC and a Z-axis drive assembly; the Z-axis drive assembly is controlled by the PLC and drives the shooting mechanism to move; the shooting mechanism comprises a camera, a point light source and a laser range finder; the laser range finder is used for measuring the distance between the laser range finder and the surface of a liquid crystal screen; the code reading mechanism is used for reading bar code information carried on the liquid crystal screen; the transverse drive device is used for determining transverse motion of the image collection device relative to the detection platform; the industrial personal computer is connected with the servo system, the PLC, the camera, the point light source and the laser range finder separately and is provided with an image collection management system and a defect identification system; and the display screen is used for displaying an operation interface and a defect detection result.