A tissue culture seedling transplanting gripper with a scratching function belongs to the field of facility agriculture automation seedling transplanting equipment, and comprises a cylinder connecting frame, a steel needle frame, a wedge bench, guide tubes, steel needles, a gripper seat, springs, a cylinder, a cylinder push rod, and movable shafts; the cylinder is mounted above the cylinder connecting frame; the gripper seat is mounted below the cylinder connecting frame; the steel needle frame and the wedge bench are fixed at the front end of the cylinder push rod; a projection is disposed at the inner side of the parallel guide tube, and the projection is provided with a hole; each of the four guide tubes is mounted on the gripper seat by the movable shaft which passes through the hole on the projection, and can rotate around the movable shaft; the four guide tubes are uniformly distributed at the periphery of the outside of the gripper seat, and are mounted parallel to the cylinderaxis. the spring is mounted at the lower part of a group of two guide tubes facing each other, and the two ends of the spring are respectively fixed on the two guide tubes; one end of one steel needle is fixed on the steel needle frame, and the other end is inserted into the guide tube. The invention is applicable to transplanting between different aperture disks, has a compact structure, and is convenient for installation and maintenance.