The invention belongs to the field of medical detection, and particularly relates to a portable biological tissue detection device based on a space deviation Raman spectrum technology. The biologicaltissue detection device comprises a computer, a detection controller, a laser, a spectrometer, a three-freedom-degree mechanical arm, an SORS detection head, a detection platform and a sealed sample chamber. An SORS technology is used; a sample to be tested is sealed; during the storage in dark places, equipment can be directly used for detecting the Raman spectrum, so that the detection is performed in a targeted way; the SORS technology and an SERS technology are used in a combined way; the defect of lower SORS detection biological sample signals is overcome; meanwhile, the problem that theSERS detection sample can only be used for detecting the expression information is solved; a novel SORS detection head is designed; the detection head has the advantages that the detection sensitivityis high; the control is easy; the displacement control precision is high; the influence by the precision of the step motor is avoided. The novel biological sample storage device is designed; the sample can be stored for a long time; the SORS signal detection can be detected at any time.