The invention discloses a dual-power isolation circuit, system and method for intelligent driving, a normally-closed relay is connected in series in a dual-power system circuit, and the opening and closing of the normally-closed relay are controlled under the opening and closing of an engine starting control signal so as to control the isolation of a main power supply and an auxiliary power supply. The circuit is simple in structure, clear in logic, few in related components, free of interference and influence of other components, convenient to maintain and capable of being modified in a short time. The relay is low in cost, can be widely applied to circuits with dual-power systems, such as the field of intelligent automobiles, large-scale equipment mounting and refitting markets, motor homes and the like, and can effectively reduce the cost. And the cost can be effectively controlled in the modification of the intelligent automobile. The circuit design has no space limitation, can be arranged together with an automobile circuit, can effectively isolate intelligent equipment, a main power supply and an automobile auxiliary power supply when the starter works, does not consume electric energy, improves the utilization rate of the electric energy, and has a stable safety characteristic.