The invention discloses a
total internal reflection microscopy device based on free-form surface shaping.The device comprises a
light source and a line polarized light generating module, a free-form surface focusing module, a
digital microscopy refection module, an optical enlargement transmission module and a
fluorescence imaging module which are sequentially arranged along a light path, wherein the line polarized light generating module is used for converting light beams emitted by the
light source into line polarized light; the free-form surface focusing module is used for converting the line polarized light into annular focused light; the
digital microscopy refection module is used for selecting annular focused light reflected in an area; the optical enlargement transmission module is used for achieving
total internal reflection illumination of the light beams; the
fluorescence imaging module is used for exciting a sample to emit
fluorescent light and collecting a
fluorescent light signal image.The invention further discloses a
total internal reflection microscopy method based on free-form surface shaping.According to the total internal reflection
microscopy method and device based on free-form surface shaping, no
mechanical vibration module is adopted, scanning is conducted more stably, and
noise is lower; the
utilization rate of
laser energy is higher, and an imaging view field is more uniform; a DMD control controls scanning, the angle is more accurate, and it is helpful to achieve layered scanning and 3D image reconstruction.