The invention relates to an extraction technology of crude
heparin sodium. The extraction technology comprises the following steps:
hydrolysis: putting broken raw materials into a reaction kettle, adding a
hydrolysis liquid into the reaction kettle, adding resin into filtrate, stirring, siphoning the supernatant liquid, collecting the resin, washing the resin in
clean water till the resin is neutral, and draining;
desorption: soaking the resin with dilute
saline water, discarding the soaking solution, adding concentrated
saline water into the resin twice, stirring, washing, filtering, and keeping the washing liquid;
alcohol precipitation: adding ethyl
alcohol into the washing liquid, stirring, carrying out standing, discarding the supernatant liquid, keeping the bottom
sediment, draining the
sediment, adding ethyl
alcohol, washing, unwatering, and collecting dehydrated
heparin sodium sediment;
drying:
drying the
heparin sodium sediment in vacuum. The extraction technology of crude
heparin sodium provided by the invention has the following advantages: all the steps are clear, so that labor division and cooperation are facilitated; the contents of various important additives have quantitative marks and are authenticated through tests, and the sequence among the additives is clear.