The invention discloses a flexible plate pressing mechanism and a plate clamping and positioning tool. The flexible plate pressing mechanism comprises a plate pressing base and at least two plate pressing assemblies, wherein a positioning sleeve is arranged on the plate pressing base, each plate pressing assembly comprises a pressing plate and an elastic plate, each elastic plate is of an arch-shaped structure, the two ends of each elastic plate act on the corresponding pressing plate, the pressing plates are installed on the positioning sleeve through connecting structures, the plate clamping and positioning tool comprises a positioning plate, a pressing plate supporting plate, an upper pulling plate and a lower pulling plate and further comprises a flexible plate pressing mechanism, the flexible plate pressing mechanism is installed on the pressing plate supporting plate through an adjusting lead screw, and a plate containing cavity is formed among the positioning plate, the upper pulling plate, the lower pulling plate and the pressing plate. The flexible plate pressing mechanism and plate clamping and the positioning tool are simple and compact in structure and convenient to operate, the position of the pressing plate is adjusted by adjusting the adjusting lead screw, the pressing plate can be completely attached to a plate, the contact area of the pressing plate and the plate is increased, the plate is evenly stressed, and stable pressing of the plate, especially the plate with the bending radian, can be achieved.