The invention provides vanadium-contained 10.9-level steel for a fastener for rail transit mobile equipment. The steel mainly comprises following chemical elements including, by mass percentage, 0.33% to 0.38% of C, 0.05% to 0.10% of Si, 0.70% to 0.90% of Mn, 0.70% to 0.90% of Cr, 0.30% to 0.60% of Ni, 0.20% to 0.30% of Mo, 0.015% to 0.045% of Als, 0.06% to 0.10% of V, 0.006% to 0.010% of [N], not larger than 0.010% of P, not larger than 0.005% of S and not larger than 0.0015% of T[O]. The autstenitic grain size of steel obtained after heat treatment is larger than 10.0 level, and according to the structure of the steel obtained after heat treatment, a small amount of small pro-eutectoid ferrite is dispersed and distributed on a tempered sorbite base body, wherein, the amount of the pro-eutectoid ferrite is smaller than or equal to 5%, and the size of the pro-eutectoid ferrite is smaller than or equal to 5 micrometers.