The invention belongs to the technical field of chemical and spectrum analysis and test, and particularly discloses a spectrophotometry quantitative analysis method of an iodine-starch color-developing system. The spectrophotometry quantitative analysis method specifically comprises the steps of: (1) in a volumetric flask, taking a quantitative I<->-containing solution to fully react with an excessive FeCl3 solution at room temperature; (2) adding a certain amount of starch solution in the volumetric flask, shaking up; (3) immediately adding distilled water in the volumetric flask for diluting until the solution is close to the neck part of the volumetric flask, shaking up; (4) immediately adding a defined amount of H2O2, shaking up, metering the volume by using the distilled water, shaking up; (5) measuring an absorption curve at 400-700nm by using FeCl3 as a blank control to determine a measurement wavelength; (6) preparing color-developing systems with different iodine concentrations by using an I<-> standard solution according to the method of (1)-(4), measuring absorbancy A by using the measurement wavelength, establishing a standard curve by using a correspondence between the I<-> concentration c and the A value, and obtaining an equation of linear regression and a coefficient of association; and (7) measuring the absorbancy A of a color-developing system of an unknown I<->-containing solution according to the method of (1)-(4), and calculating the concentration of I<-> in the system to be measured according to the equation of linear regression in the (6). The spectrophotometry quantitative analysis method is stable in color-developing system, can be directly and indirectly used for iodine amount analysis, has the advantages of convenience, accuracy, trace amount, rapidness, low cost and the like, and can be widely applied.