The invention discloses a constant temperature and humidity cell culture box. The incubator of the present invention includes a box body, a box door, a chamber, a water heating mechanism, a humidifying plate, a support rod, a humidity maintaining mechanism, and a CO 2 Generator, No. 1 Exhaust Pipe and CO 2 Concentration maintenance mechanism, one side of the box body is movably connected to the box door, there are three chambers with equal volume inside the box body, a water heating mechanism is installed in the side wall of the box body, each chamber is equipped with a humidifying plate, and the middle of each chamber Both ends are fixedly connected to one end of the support rod, and the other end of the support rod is fixedly connected to the humidification plate. The humidification plate is a cube with an opening on one side. The inside of the box is equipped with a humidity maintaining mechanism. 2 Generator, one end of No. 1 exhaust pipe is fixedly connected to CO 2 Generator, No. 1 exhaust pipe away from CO 2 One end of the generator is placed on the upper end of the box, and the inside of the box is equipped with a CO 2 Concentration maintaining mechanism, the box door is made of transparent material, and the box door is coated with light-shielding material. The invention is scientific and reasonable, simple in structure, and economical.