The invention provides an anti-fatigue cushion intelligent control unit. The intelligent control unit comprises a one-chip microcomputer control module, a temperature sensor module, a human body capacitance sensor module, an alarm module, a massage module, a timer module, a heating physiotherapy module and a refrigeration and heat radiation module, and the one-chip microcomputer control module is respectively connected with a power supply module, a pressure sensor module, the temperature sensor module, the human body capacitance sensor module, the alarm module, the timer module, the heating physiotherapy module, the refrigeration and heat radiation module and a manual setting module. A temperature sensor collects temperature data of surrounding environment and a cushion and human body contact surface, the heating physiotherapy module and the refrigeration and heat radiation module provide a most comfortable temperature for a user, the timer module records the sitting time of the user, the alarm module regularly prompts the user to depart from the cushion and properly take a rest, and the massage module can provide basic massage, so the anti-fatigue cushion intelligent control unit has the advantages of convenient and safe use, and alleviation of fatigue induced by long-time sitting.