The application belongs to the technical field of Raman
spectrum analysis, and particularly relates to an
optical flow Raman sensing
chip based on a SERS substrate and a preparation method thereof. The
optical flow Raman sensing
chip comprises a first PDMS layer, a
silicon-based optical
waveguide layer and a second PDMS layer, wherein the
silicon-based optical
waveguide layer is arranged on the top surface of the first PDMS layer, the
silicon-based optical
waveguide layer has a top surface and two side surfaces, the second PDMS layer is arranged on the top surface and the side surfaces of the silicon-based optical waveguide layer, the surface of the side surface of the second PDMS layer facing the silicon-based optical waveguide layer is provided with a near-
coaxial cavity, and the arc-shaped inner wall of the near-
coaxial cavity is provided with a reflecting film. The target object solution is specifically combined with the SERS composite layer through the
microfluidic channel, the SERS composite layer loaded with the target object generates
Raman laser under the excitation of pump light, and the
Raman laser is output after enhancement under the synergistic effect of the arc-shaped inner wall and the reflecting film. The application significantly improves the collection efficiency of the Raman
signal through the multi-dimensional
coupling enhancement effect of the cavity structure.