The invention discloses a test case set generation method based on a combination chaotic sequence; the method comprises the following steps in sequence: a, using Chebyshev mapping and Logistic mapping to combine, thus forming the chaotic sequence; b, using the chaotic sequence to initialize population; c, setting an individual fitness function; d, defining a heredity operator; e, adding chaotic disturbance. The method uses the combination chaotic mapping to generate the initial population, each population individual comprises n genes, the population individual is a possible test case selection scheme, the combination chaotic sequence with uniform distribution characteristic is introduced to heredity algorithmic selection, intersect and variation operation, thus effectively preventing immature convergence, improving algorithmic global search capability, and calculating efficiency chaotic system generation initial population genes, adding chaotic small disturbance to each chaotic variable so as to carry out population optimization, and restraining to a proper individual through continuously evolution; test case minimization is converted into a method in which the individual with least genes is searched in the population, thus covering all test needs.