The invention discloses a gynecological and obstetric nursing disinfectant and a preparation method thereof, and belongs to the technical field of gynecological and obstetric nursing. The disinfectantis prepared from the following raw material components in parts by weight: 15-26 parts of a traditional Chinese medicine extract, 0.5-2 parts of glycerol, 0.2-1 part of an antibacterial agent, and 100-150 parts of sterile water; and the pH value is adjusted to 3.8-4.2 by citric acid or sodium hydroxide. The traditional Chinese medicine extract is prepared from the following raw material medicinesin parts by weight: 25-36 parts of motherwort, 16-25 parts of cortex phellodendri, 10-20 parts of fructus cnidii, 16-28 parts of honeysuckle, 10-18 parts of radix sophorae flavescentis, and 8-16 parts of rhizoma coptidis. In addition, the invention also discloses the preparation method of the disinfectant. The traditional Chinese medicine extracts of motherwort, cortex phellodendri, fructus cnidii, honeysuckle, radix sophorae flavescentis, rhizoma coptidis and the like with complemented drug properties are combined with glycerol and the antibacterial agent, and the gynecological and obstetricnursing disinfectant with bactericidal, anti-inflammatory, heat-clearing, dampness-drying, itching-relieving and pain-relieving effects is prepared; the pH value is adjusted to 3.8-4.2 by citric acidor sodium hydroxide, so the nursing disinfectant can regulate the balance of acidity and alkalinity in private places, avoids bacterial breeding, and is safe to use, strong in nursing, and worthy ofpopularizing and using widely.