The invention belongs to the field of analytical chemistry, and particularly relates to a method for separating and determining levocetirizine hydrochloride and enantiomer thereof through an HPLC method. In the method, an adopted chromatographic column takes silica gel of which the surface is chemically bonded with beta-cyclodextrin as filler, elution is conducted through a mobile phase A and a mobile phase B, and detection is conducted by entering a detector; the mobile phase A is an inorganic salt buffer system, and the mobile phase B is organic solvent. The method is a reversed phase high performance liquid chromatography method, separation and determination of levocetirizine hydrochloride and the enantiomer thereof can be achieved simultaneously, excellent separation performance and durability are achieved, the method is simple, convenient, feasible, good in reproducibility, efficient and rapid, the analysis time is significantly shortened, the tailing factor and theoretical pedal number can both reach very good effects, the content of the enantiomer in levocetirizine hydrochloride can be effectively determined, and specificity is high.