The invention relates to an on-line control method of liquid nitrogen injection quantity for ultra-low temperature cooling processing. The on-line control method of the liquid nitrogen injection quantity for ultra-low temperature cooling processing comprises the following steps that firstly, a relation model between cutting process parameters and the liquid nitrogen injection quantity is constructed, and the model is placed into an on-line controller for ultra-low temperature processing liquid nitrogen injection. The controller can regulate and control material characteristics, tool parameters, an optimal cutting temperature and heat dissipation ratio coefficients input by a central control computer of a liquid nitrogen supply device based on the collected cutting process parameters of a machine tool numerical control processing system, and expected liquid nitrogen injection quantity is determined by calling the relational model and a parameter database; and meanwhile, the controller collects the temperature of a cutting area online through a thermocouple fixed in an internal jet type cutting tool to feedback and adjust actual liquid nitrogen injection quantity to change within a certain range, so that the cutting area is in an optimal cutting temperature range, the processing quality of parts is ensured, and the service life of the cutting tool is prolonged. The on-line control of the liquid nitrogen injection on-line quantity is achieved according to the heating condition of cutting processing, optimization iteration time is short, and accurate control over the temperature of the cutting area can be quickly realized.