The invention discloses a flocculating agent for cement high pressure jet grouting and preparation method and application thereof. The flocculating agent is composed of konjac glucomannan, carbonate, rheological agent, dispersing agent, early strength agent and water by uniform stirring, distillation and smashing, and the weight ratio of the konjac glucomannan, carbonate, rheological agent, dispersing agent and early strength agent is 35-45: 22-28: 8-12: 13-17: 8-12; the preparation thereof includes the steps: raw material weighing and fully dissolution, mixed slurry confecting and distillation, spray drying, smashing and sieving; and the application thereof includes the steps: admixture determination, flocculating agent cement slurry confecting and slurry grouting completing by utilizinghigh pressure jet grouting process. The invention has simple preparation step, low cost and simple and convenient use and has no harm on human body, application in high pressure jet grouting construction is simple and convenient and processing effect is good, and the problems that pure cement slurry has poor adhesion, high cement slurry drain, high construction cost and influence on construction progress existed in high pressure jet impermeable grouting can be solved.