The invention belongs to a honeycomb preparation technique, and relates to a preparation method of honeycomb core materials for seamless splicing. According to the method, nonmetal honeycombs, which are same in material and size, are selected to be spliced, and edge cutting treatment is conducted on spliced edges of the honeycombs; then, notch pre-treatment is conducted on the honeycombs to be spliced along splicing lines separately to make the inner walls, at the spliced edges, of the honeycombs remain in an open state, and the inner sides of the spliced edges of the honeycomb notches are coated with node glue; the openings at the two ends of each spliced edge are embedded and spliced, the notches coated with the node glue at the two ends are bonded with each other to form an integral honeycomb grid at a spliced place, and the spliced edges are fixedly pasted by metal wires; finally, the honeycombs after splicing are put into an oasthouse for heating treatment, after solidification iscompleted, the honeycombs are cooled to the room temperature, the metal wires are removed, and seamless solidification of the spliced edges of the notches coated with the glue is finished. Through utilization of grid features of the honeycomb core materials, the spliced notches are designed, and under the cooperation of a fixing effect of the metal wires, seamless bonding and splicing of the honeycomb core materials are completed; the integral continuity of the spliced honeycombs is maintained to the maximum degree.