The invention belongs to the field of pharmaceutical preparations, and provides an
andrographolide nano suspension and a preparation method thereof. The
andrographolide has low
solubility in water, can be easily excreted by P-gp, has high
metabolism rate, and thus, has lower
dissolution rate and lower in-vivo
bioavailability. In order to enhance the
dissolution rate and
bioavailability of the
drug, a P-gp inhibitor TPGS and an ionic stabilizer SDS are selected to prepare the
andrographolide nano suspension by using a medium
grinding technique; by using the particle size and distribution as evaluating indicators, a Box-Behnken center combined degisn test optimized preparation technique is utilized to prepare the andrographolide nano suspension; and freeze-
drying is used for solidifying. After carrying out the characterization, the research in short-term stability, Caco-2 permeability and in-vitro
dissolution, and the in-vivo pharmacokinetic and pharmacodynamic research, the results indicate that the andrographolide nano suspension can increase the dissolution rate of andrographolide and enhance the oral-administration
bioavailability and anti-inflammatory action.