The invention provides an optical line terminal, a passive optical network and a radio frequency signal transmission method, and pertains to the technical field of communication. The passive optical networks comprises an OLT, an ODN and at least one ONU, wherein, the OLT comprises at least one transmission unit which provides a downstream optical carrier and two upstream optical carriers dedicated to the ONU, modulates a downstream radio frequency signal to be sent to the ONU to a downstream optical carrier dedicated to the ONU, and outputs a downstream optical signal after mixing the modulated upstream optical carrier and the corresponding two dedicated upstream optical carriers, wherein, the two upstream optical carriers dedicated to the ONU is used for carrying the upstream radio frequency signal of the ONU; a multiplexing / demultiplexing unit which is used for transmitting the downstream optical signals output by various transmission units to the ONU by the ODN after the wavelength division multiplexing, and demultiplexing and outputting the upstream optical wavelength division of various ONUs which are subject to the wavelength division multiplexing; and at least one receiving unit which is used for obtaining an upstream signal from the demultiplexed upstream optical signal. The bandwidth of a radio access network is enlarged, and the ONU has simple design.