The invention discloses dried beef with the function of enhancing the immunity. The dried beef comprises the following raw materials: beef, table salt, soy sauce, white granulated sugar, monosodium glutamate, olive wine, chili sauce, raw ginger, spice, olive leaves and dried olive fruits. According to the dried beef disclosed by the invention, nutritional ingredients of the olive leaves, the olive fruits and the olive wine, especially the active ingredients, such as Oleuropein and hydroxytyrosol in the olive leaves are sufficiently utilized and are absorbed by the dried beef, so that the dried beef achieves the health effects of enhancing the immunity and the like and simultaneously has the functions of enriching nutrition, resisting influenza and bacteria, adjusting the immunity of the human body and the like; especially, the finished-product dried beef is fine in mouthfeel, high in integrity and long in shelf life, and has few slag; meanwhile, a preparation method of the dried beef has the characteristics of being simple, free of special equipment, low in cost and the like.