The invention discloses a seedling cultivation device which comprises a base, wherein wheels are fixedly connected to the two sides of the bottom of the base, a push rod is fixedly connected to the top of the base, a water storage tank is fixedly connected to the middle of the top of the base, a fixing ring is fixedly connected to the position, located on the bottom surface of the water storage tank, of the top of the base, a water inlet is formed in the bottom of the front face of the water storage tank, and a cultivation device is arranged at the top of the water storage tank. The invention relates to the technical field of agricultural planting. According to the seedling cultivation device, the cultivation device, the fixing ring and the water storage tank are arranged, the cultivation device supplements water through the water storage tank, quantitative spraying is achieved, water waste is avoided, the device is convenient to move, the parking position of the device is convenient to adjust, seedlings are not prone to damage when taken out, recycled resources are not easily polluted and convenient to reuse, the situation that illumination is affected when the device is folded is avoided, the temperature is controlled, and growth of seedlings is facilitated.