The invention discloses a pneumatic micro-operation device for tiny organisms, which comprises a controller, a negative pressure vacuum pump, a negative pressure throttle valve, a vacuum proportional valve, an adsorption electromagnetic valve, a pressure sensor, a micro-attitude adjustment component, a positive pressure gas pump, a positive pressure throttle valve, a positive pressure proportional valve, a release electromagnetic valve, a speed control valve and a tail-end micro-clip. The pneumatic micro-operation device can be divided into a negative pressure adsorption gas path and a positive pressure release gas path, thereby carrying out adsorption micro-operation and release micro-operation. The pneumatic micro-operation device has the advantages that the operation tail end has the micro-attitude adjustment function and can realize sorting, screening and other micro-operations of the tiny organisms without injuring and damaging the organisms, and the pneumatic micro-operation device can improve the efficiency and the success rate of operation staff, reduce the working strength and have broad practical application prospects and value by being applied in the field of biomedicalresearch.