The invention discloses an affinity prediction and 
analysis method for a human amphiphysin-1 Src homology-3 domain 
binding peptide. The method comprises the following steps of: selecting an electrical property parameter, a three-dimensional property parameter, a hydrophobicity parameter and 
hydrogen bond property parameter of an 
amino acid, establishing physicochemical property classification scores of the 
amino acid by adopting a 
principal component analysis method, representing each 
amino acid residue in 
a peptide sequence by using the scores to realize the parameter-based description of 
a peptide sequence structure, selecting characteristic parameters closely related to affinity from parameters of the 
peptide sequence structure by using a 
genetic algorithm, establishing a 
relational model, namely an affinity prediction and analysis model between the characteristic parameters and the affinity by using a 
Gaussian process, obtaining structural factors influencing the affinity of the 
peptide by analyzing hyper-parameters of the model, and performing prediction by using the model to obtain an affinity value of the 
peptide. By the method, a powerful tool can be provided for the high-
throughput screening of high-affinity human amphiphysin-1 Src homology-3 domain binding peptides, and development and researches on human amphiphysin-1 Src homology-3 domain 
binding peptide inhibitors are further prompted.