The invention discloses a thrust rack in-situ calibration system, which comprises a standard sensor assembly, a hydraulic system and a loading and unloading control system, wherein the standard sensor assembly comprises a sensor pull rod, a front universal flexible element, a standard sensor and a back universal flexible element, the hydraulic system comprises a hydraulic cylinder, a press cavity oil filling valve, a hand lifting pump, a pulling cavity oil filling valve, an electric joint pressure meter and a pressure cavity energy accumulator, the loading and unloading control system comprises a self control pump, a wide frequency servo motor, a pulse automatic tracking system, a computer and a collecting card, the in-situ thrust calibration system controls the work of the self control pump through the pulse automatic tracking system and the wide frequency servo motor, in addition, measurement signals of the standard sensor are fed back to the computer, the computer transmits the pulse signals to the pulse automatic tracking system through software operation, the automatic loading and unloading on the standard sensor by the hydraulic cylinder is realized, in addition, the necessary time can be stabilized, the loading stability can reach 0.004 percent F.S per 30 minutes (or longer time).