49 results about "Increased leukocytes" patented technology

The invention discloses a sea cucumber polypeptide, wherein a preparation method thereof comprises the following steps: (1) getting fresh stichopus japonicus, removing entrails, cleaning and crushing the stichopus japonicus into small blocks, and adding water to form homogenate; after the enzymolysis of the homogenate liquid, centrifugating and getting the supernatant; adding ethanol, standing, centrifugating and getting the supernatant, concentrating the supernatant at reducing pressure and drying the concentrate to form a crude product of the sea cucumber polypeptide; (2) dissolving the crude product of the sea cucumber polypeptide by using distilled water, carrying out gel filtration chromatography by Sephadex LH-20, eluting the sea cucumber polypeptide by double-distilled water, getting a second peak, collecting active components, and condensing, freezing and freeze-drying the active components; and (3) dissolving the active components by double-distilled water, carrying out ion exchange chromatography by Q Sepharose Fast Flow, linearly eluting the active components by NaCl solution, desalting, getting the second peak, collecting the active components, and condensing, freeze-drying the active components to form the sea cucumber polypeptide. The sea cucumber polypeptide can be used for preparing medicaments or health-care products for increasing leukocytes and also can be used for preparing medicaments or health-care products for multiplying marrow cells after chemotherapy.

The health food consists of liquid material and solid material, the liquid material is compounded with donkey-hide gelatin, crystal sugar, yellow wine and honey, and solid material is produced with pitted date, walnut kernel, sesame, wolfberry fruit, pitted and shelled longan, ginseng, American ginseng, lily, coix seed and tremella. The production process of the health food includes soaking donkey-hide gelatin and crystal sugar in yellow wine for 5-7 days; crushing the solid material; mixing the solid material and liquid material; steaming the mixture for 3 hr while stirring; and packing while the steamed mixture is still hot. The health food has the functions of invigorating vital energy, nourishing blood, increasing leukocytes, etc.

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. They can also be used for increasing white blood cell survival and for chemotherapy.

The present invention relates to medicine composition extracted from Paeonia lactifora Pall and containing the active components paeoniflorin and albiflorin in the total content of 50-95 % and weight ratio of 0.1-10. The composition is suitable for being prepared into various medicine preparations, including delayed releasing preparation, controlled releasing preparation, body cavity administrated preparation, spray, transdermal preparation, oral preparation, injection and transfused fluid preparation alone or together with other medicine components. The composition is suitable for preparing medicine for treating leucopenia, throbocytopenia and achreocythemia of different causes.

An object of the present invention is to provide a method and device for filtering blood or blood components which simultaneously attains 1) efficiently increasing leukocyte removal capability and 2) effectively utilizing the filtration area by preventing nonuniform flow, when blood is filtered by being fed to a leukocyte-removing filter comprising flexible containers by being pressurized using a pump or the like. The method comprises providing a leukocyte-removing filter having a thickness of 9.0 mm to 9.4 mm, an air permeability pressure drop of 300 Pa to 700 Pa, and effective filtration area of 50 cm2 to 70 cm2 which comprises a flat and flexible container having an inlet port and an outlet port and a sheet-like leukocyte-removing-filter material with a thickness of 8.2 mm to 8.6 mm disposed to divide the container into an inlet-port side chamber and an outlet-port side chamber, providing a pair of volume restriction boards disposed outside of the inlet port side external surface and the outlet port side external surface of the flexible container, having the distance between the volume restriction boards at the portion where the volume restriction boards facing each other sandwich the leukocyte-removing filter being in a range from [thickness of leukocyte-removing filter+0.5 mm] to [thickness of leukocyte-removing filter+2.5 mm], and filtering blood or blood components through the leukocyte-removing filter under a pressure of 30 kPa to 50 kPa while restraining expansion of the flexible container occurring during filtration by means of the volume restriction boards.

The invention belongs to the fileds of chemistry and pharmacy, in particular to caffeic acid p-nitrophenyl ethyl ester of which the structural formula is as shown in formula I. The caffeic acid p-nitrophenyl ethyl ester has the effect of increasing leukocytes, can obviously increase the number of the peripheral blood leukocytes of mice with immunosuppression caused by cyclophosphamide, can be used for preparing medicaments for increasing the white blood cells, plays an important role in treating leukopenia after radiochemotherapy on malignant tumors and possibly has a good curative effect on the leukopenia caused by other pathogenic factors. The invention also provides a preparation method of the caffeic acid p-nitrophenyl ethyl ester, which comprises the following steps: heating caffeic acid and thionyl chloride in a polar organic solvent to carry out a refluxing reaction so as to generate caffeic acid acyl chloride, adding p-nitrophenyl ethanol, continuing to heat to carry out the refluxing reaction so as to generate the caffeic acid p-nitrophenyl ethyl ester, and finally separating and purifying by column chromatography to obtain pure caffeic acid p-nitrophenyl ethyl ester. Themethod has the advantages of high yield and low cost, is convenient to operate and the like.

Methods to improve hematopoiesis and increase white blood cell counts in subjects and patients using pyrimidine-based inhibitors of Bruton's tyrosine kinase (Btk) are disclosed.

The invention provides nutrient solution with the advantages of rich nutrition, easy absorption and comprehensive effects. The nutrient solution is prepared from astragalus, chinaroot greenbrier rhizome, peanut kernel, Chinese data and medlar serving as raw materials. A method for preparing the nutrient solution comprises the following steps of: after cleaning the raw materials, cutting the astragalus and crushing the rest components; adding water, mixing and boiling for 20 to 30 minutes; and filtering to obtain the nutrient solution, and sterilizing and preserving. The nutrient solution after being taken for a long time has the effects of adjusting physique, obviously improving and enhancing the immunity of patients with tumor, increasing leukocytes, inducing interferons, conditioning body functions, enhancing detoxifying and detoxicating capability, and has the advantages of scientific compatibility and rich nutrition. The nutrient solution well keeps nutritional ingredients by adopting ultrahigh temperature (UHT) instantaneous sterilization, and has the advantages of low cost, simple and easy preparation method, and obvious economic and social benefits.

Methods to improve hematopoiesis and increase white blood cell counts in subjects and patients using pyrimidine-based inhibitors of Bruton's tyrosine kinase (Btk) are disclosed.

The invention discloses a method for preparing a five-element vegetable soup base, which comprises the following steps: (1) material taking, cleaning and sized dicing; (2) blanching; (3) drying; (4) mixing and stirring; and (5) sieving, magnetic inspection passing, metallurgy inspection passing, and packaging. In case of often drinking soup prepared by using the five-element vegetable soup base disclosed by the invention, the effects of promoting regular propagation, increasing leukocytes and thrombocytes in blood, and increasing somatic cells at three times speed are achieved, thereby achieving the purpose of improving human immunity, preventing diseases and keeping healthy.

The invention belongs to the field of traditional Chinese medicines, and relates to glycopeptide extract from ganoderma spore powder, a method for extracting the glycopeptide extract and application thereof. The method sequentially includes steps of extracting the ganoderma spore powder by the aid of concentrated alcohol; extracting the ganoderma spore powder by the aid of diluted alcohol; combining concentrated-alcohol extract liquid and diluted-alcohol extract liquid with each other to obtain mixed extract liquid and degreasing the mixed extract liquid by the aid of diethyl ether; removing impurities from the mixed extract liquid and decolorizing the mixed extract liquid; purifying the mixed extract liquid by the aid of resin. The method preferably further includes steps of further treating ganoderma spore powder residues by the aid of aqueous extraction, alcohol precipitation and refining steps. The ganoderma spore powder residues remain after the ganoderma spore powder is extracted by the aid of the concentrated alcohol and the diluted alcohol. The glycopeptide extract is prepared by the aid of the method. The glycopeptide extract, the method and the application have the advantages that the method for extracting the glycopeptide extract from the ganoderma spore powder is easy and convenient to implement and low in cost and is environmentally friendly and safe, good extraction effects can be realized, structures of the glycopeptide extract can be prevented from being destroyed, solvents can be recycled, and industrial production can be facilitated; excellent effects can be realized by the glycopeptide extract when the glycopeptide extract is used for increasing leukocytes.

The invention discloses a biological reaction regulator capable of promoting hematopoietic function and enhancing immunity, preparation and application thereof. The biological reaction regulator is prepared from the following traditional Chinese medicine raw materials in parts by weight: 10-30 parts of squid ink, 5-30 parts of anoectochilus roxburghii, 5-10 parts of xylariasp, 10-30 parts of dendrobium candidum, 10-30 parts of radix rehmanniae praeparata, 10-30 parts of wolfberries, 10-30 parts of semen cuscutae, 10-30 parts of radix astragali, 10-30 parts of angelica sinensis and 10-30 parts of liquorice. A preparation method of the biological reaction regulator comprises the following steps: mixing the traditional Chinese medicine raw materials, then adding water for immersing for 1.5-3 hours, heating and decocting, then pouring decoction liquid, adding water, heating, decocting and pouring decoction liquid, combining the decoction fluids and filtering to obtain clear liquid, concentrating, spray drying, crushing and screening to obtain the biological reaction regulator capable of promoting hematopoietic function and enhancing immunity. The biological reaction regulator has remarkable effects of protecting leukocyte, promoting differentiation of hematopoietic stem cells and increasing leukocyte.

The invention discloses a hematopoietic stem cell mobilization agent. The hematopoietic stem cell mobilization agent is prepared from conyza japonica saponin R, and can be further prepared from dexamethasone, HS6101 and the like. CD34 is selectively expressed on the surfaces of early hematopoietic stem / progenitor cells and is the main marker for hematopoietic stem cell isolation and purification.If the number of hematopoietic stem cells in peripheral blood is increased, the number of white blood cells can also be increased. Therefore, the number of white blood cells in the peripheral blood can serve as an index reflecting the content of the hematopoietic stem cells in the peripheral blood. It is found through experiments that injection of the conyza japonica saponin R or dexamethasone cansignificantly increase the content of the white blood cells in the peripheral blood, and the combination of drugs had a synergistic effect. A brand-new hematopoietic cell mobilization agent is found,the toxic and side effects are low, the action effect is good, and the agent has a remarkable synergistic effect with other drugs.

The invention relates to a composition with the effect of promoting leukopoiesis and a preparation method thereof. The preparation method comprises the following steps: mixing crude drugs which are radix astragali, fructus chaenomelis, poria cocos and radix angelicae sinensis to obtain a mixture, extracting twice with water 10-20 times the amount of mixture, every time being 2 hours, combining andextracting the extracting liquor into paste, vacuum-drying, crushing and screening; adding crushed and screened mixture of colla corii asini and radix ginseng, and adding royal jelly to pelletize. The traditional Chinese medicine composition disclosed by the invention has obvious effects of increasing leukocyte and improving immunity, further can assist and inhibit cancer cell increase, and has remarkably reduced toxic and side effects in comparison with that of a leukocyte increasing drug on the market.

The invention relates to the field of organic synthesis and medical chemistry, particularly relates to a composition, a preparation method of the composition and application of the composition in preparing a medicine for increasing leukocyte, and discloses a composition and a preparation method thereof. Pharmacological experiments indicate that the composition has an effect of increasing leukocyte, and has a value of developing medicines for increasing leukocyte.

The invention provides the application of an astragalus membranaceus flavonoid extract to preparation of medicines for treating leukopenia. The astragalus membranaceus flavonoid extract has the flavonoid content of 40-60%, and comprises the following main chemical components: formononetin, ononin, calycosin, calycosin-7-O-beta-D-glucopyranose, 7, 2'-dihydroxy-3', 4'-dimethoxyisoflavane, 2'-hydroxy-3', 4'-dimethoxyisoflavan-7-O-beta-D-glucopyranose, 3-hydroxy-9, 10-dimethoxypterocarpane and 9,10-dimethoxypterocarpane-3-O-beta-D-glucopyranose. The astragalus membranaceus flavonoid extract has arelatively good effect of increasing leukocytes and can be applied to preparation of the medicines for treating or preventing the leukopenia or auxiliary chemoradiotherapeutic medicines.

The invention relates to the fields of organic synthesis and pharmaceutical chemistry, and specifically relates to a cleistanone O-(benzimidazolyl) ethyl derivative, a preparation method thereof, and an application of the cleistanone O-(benzimidazolyl) ethyl derivative in the preparation of drugs for increasing the leukocyte. A novel cleistanone O-(benzimidazolyl) ethyl derivative is synthesized, and a preparation method thereof is disclosed. The pharmacological experiments show that the cleistanone O-(benzimidazolyl) ethyl derivative has an effect on increasing leukocyte and can be used to develop drugs for increasing leukocyte.

The invention discloses a fully automatic moxibustion bed for increasing leukocytes, comprising a bed inner chamber, wherein a bed panel is arranged at the upper end of the bed inner chamber. The invention also comprises a fully automatic moxibustion device, wherein the fully automatic moxibustion device is arranged in the bed cavity; The fully automatic moxibustion device is divided into a plurality of moxibustion components. The bed panel is provided with a plurality of openings. The moxibustion assemblies are respectively arranged at the lower ends of a plurality of corresponding openings;The moxibustion assembly comprises a driving mechanism, wherein the driving mechanism is connected with a guide rail, a rotating disk is installed on the guide rail, a needle body for placing a moxa bar is arranged on one side of the rotating disk, and the needle body and the rotating disk surface form a first included angle; At that low end of the rotating disk, a collection box is arranged. Therotating disk is also provided with a monitoring mechanism; The driving mechanism, the rotating disk and the monitoring mechanism are connected with the control system. The invention has the advantages of full automation, effective promotion of white blood cells and the like.

The invention relates to the field of medicine, and in particular, relates to a medical application of icaritin. The icaritin disclosed by the invention can be used for preventing and treating myelosuppression, increasing leukocytes and / or platelets, promoting proliferation of bone marrow hematopoietic stem cells, promoting proliferation of bone marrow mesenchymal stem cells, promoting osteogenic differentiation of the bone marrow mesenchymal stem cells and inhibiting adipogenic differentiation of the bone marrow mesenchymal stem cells.

The invention relates to the field of organic synthesis and medicinal chemistry and in particular relates to a composition, a preparation method and an application of the composition to preparation of medicines capable of increasing leukocytes. The invention discloses the composition and the preparation method thereof. Pharmacological experiments show that the composition has the function of increasing leukocytes and has the value in developing the medicines capable of increasing leukocytes.

The medicine for increasing leukocyte comprises 10 Chinese medicinal materials of turtle shell, millettia root and stem, ginseng, prepared flowery knotweed root, jujube and others. Its preparation process includes the following steps: (1). drying and pulverizing turtle shell to obtain fine powder; (2) making millettia root and stem into millettia paste; and (3) making other ingredients into oral liquor, said three portions are formed into the medicine with the functions of raising physiological function and immunity of human body and a certain anticancer action. It has no any adverse reactions.

The beer with added vitamin beneficial to human body is produced through adding selected vitamins into beer via stirring for 5-60 min, letting stand to age for 10 min to 180 days at ¿C10 deg.c to 50 deg.c for vitamins to be dissolved in beer, and filtering with diatomite to eliminate insoluble matter. The vitamins include vitamin A for maintaining normal vision; vitamin B1 for maintaining the normal function of heart, nerve and digestive system; vitamin B2 for antioxidant defense; vitamin B4 for increasing leukocyte in blood; vitamin B6 for metabolism of amino acid and fat; vitamin B12 for synthesis of nucleic acid; vitamin C for bodyí»s sugar metabolism and oxidation-reduction process; vitamin D to promote the absorption of calcium and phosphate; vitamin E with antioxidant effect; and vitamin M for preventing and treating malnutrition and anemia.

The invention discloses an immune adjustment preparation for treating chronic periodontitis and a preparation method of the immune adjustment preparation for treating chronic periodontitis, and belongs to the technical field of periodontitis treatment medicines. The preparation comprises the following components in parts by weight of 4-10 parts of radix et rhizoma rhei, 6-12 parts of radix scutellariae, 10-16 parts of fructus forsythiae, 6-12 parts of heartleaf houttuynia herbs, 5-14 parts of honeysuckle flowers, 5-11 parts of spina gleditsiae, 6-12 parts of herba violae and 5-10 parts of licorice roots. The preparation method comprises the following steps of respectively crushing raw materials to obtain fine powder, performing uniform mixing in parts by weight, adding ethanol, performingheating and reflux extraction for 1-3 times, combining extracting solutions, performing filtration, performing concentration, removing ethanol, obtaining paste, performing crushing to obtain powder, and performing screening with a 20-mesh sieve so as to obtain the immune adjustment preparation. According to the immune adjustment preparation disclosed by the invention, the heartleaf houttuynia herbs, the honeysuckle flowers and the fructus forsythiae have the efficacy of resisting bacteria, so that the capacity of a product medicine for restraining pathogenic bacteria can be increased, and thepossibility that organisms are easily influenced by other viruses due to reduction of immunity of the organisms at the period of tooth inflammation can be reduced; and besides, the heartleaf houttuynia herbs also have the treatment effect of reinforcing the immune functions of the organisms, so that the swallowing capacity of leucocyte can be increased, and the treatment effects of the product medicine can be increased.

The invention discloses an application of a composition of Salviskinone A benzimidazolyl and di(2-methylmercapto ethyl)amido derivatives in drugs for increasing leukocytes, relates to the fields of organic synthesis and medicinal chemistry, and particularly relates to the composition of the Salviskinone A benzimidazolyl and di(2-methylmercapto ethyl)amido derivatives, and a preparation method and the application thereof in preparation of the drugs for increasing the leukocytes. Provided are the composition of the Salviskinone A benzimidazolyl and di(2-methylmercapto ethyl)amido derivatives and the preparation method thereof. Pharmacological experiments indicate that the composition has an effect of increasing the leukocytes, and has the value for development of the drugs for increasing the leukocytes.

The invention belongs to the fileds of chemistry and pharmacy, in particular to caffeic acid p-nitrophenyl ethyl ester of which the structural formula is as shown in formula I. The caffeic acid p-nitrophenyl ethyl ester has the effect of increasing leukocytes, can obviously increase the number of the peripheral blood leukocytes of mice with immunosuppression caused by cyclophosphamide, can be used for preparing medicaments for increasing the white blood cells, plays an important role in treating leukopenia after radiochemotherapy on malignant tumors and possibly has a good curative effect on the leukopenia caused by other pathogenic factors. The invention also provides a preparation method of the caffeic acid p-nitrophenyl ethyl ester, which comprises the following steps: heating caffeic acid and thionyl chloride in a polar organic solvent to carry out a refluxing reaction so as to generate caffeic acid acyl chloride, adding p-nitrophenyl ethanol, continuing to heat to carry out the refluxing reaction so as to generate the caffeic acid p-nitrophenyl ethyl ester, and finally separating and purifying by column chromatography to obtain pure caffeic acid p-nitrophenyl ethyl ester. Themethod has the advantages of high yield and low cost, is convenient to operate and the like.

The invention discloses a method for constructing a spontaneous anemia mouse model with leukocytes and thrombocytosis. The method is to mutate the 177th and 180th lysines of the mouse STAT3 protein into arginines. In the present invention, by performing site-directed mutation on the STAT3 gene, the 177th and 180th lysines of STAT3 are site-directedly mutated into arginine, and it is found that the mice show that white blood cells including lymphocytes, granulocytes, and monocytes are all increased, and platelets Anemia models with elevated but significantly reduced red blood cells and hemoglobin are also shown. The mouse model of the disease established in the present invention is of great significance for the study of the occurrence and development mechanism of anemia accompanied by the increase of white blood cells and platelets and the development of anti-drugs.

The invention provides nutrient solution with the advantages of rich nutrition, easy absorption and comprehensive effects. The nutrient solution is prepared from astragalus, chinaroot greenbrier rhizome, peanut kernel, Chinese data and medlar serving as raw materials. A method for preparing the nutrient solution comprises the following steps of: after cleaning the raw materials, cutting the astragalus and crushing the rest components; adding water, mixing and boiling for 20 to 30 minutes; and filtering to obtain the nutrient solution, and sterilizing and preserving. The nutrient solution after being taken for a long time has the effects of adjusting physique, obviously improving and enhancing the immunity of patients with tumor, increasing leukocytes, inducing interferons, conditioning body functions, enhancing detoxifying and detoxicating capability, and has the advantages of scientific compatibility and rich nutrition. The nutrient solution well keeps nutritional ingredients by adopting ultrahigh temperature (UHT) instantaneous sterilization, and has the advantages of low cost, simple and easy preparation method, and obvious economic and social benefits.

The invention relates to the field of organic synthesis and medicinal chemistry and in particular relates to a composition, a preparation method and an application of the composition to preparation of medicines with the function of increasing leukocytes. The invention discloses the composition and the preparation method thereof. Pharmacological experiments show that the composition has the function of increasing leukocytes and has the value in developing the medicines with the function of increasing leukocytes.