The invention relates to a method for preparing natural material inorganic nanoparticle composite nano-fiber through electrostatic spinning, which comprises steps that a template is added into diluted aqueous alkali, the mixture is heated to 80-90 DEG C through an oil bath, mixed for 1-10 hours, gradually added with tetraethyl orthosilicate, centrifugally washed by deionized water and absolute ethyl alcohol sequentially, and dried and reflowed in HCL-ethanol mixed liquor to remove the template, so freeze dried mesoporous silicon is obtained; and natural materials are dissolved in the solvent to prepare the natural material solution, the mesoporous silicon is added into the natural material solution for electrostatic spinning, fumigating and crosslinking are carried out for 0-48 hours, so the natural material mesoporous silicon composite nano-fiber is obtained. The natural material inorganic nanoparticle composite nano-fiber which is prepared by the method has good biocompatibility, a simple preparation method and moderate reaction conditions, is low in experimental raw material price, has high drug-loading rate, can be controlled to be released for a long term, and can be applied in the biomedicine and tissue regeneration fields of growth factor and gene release of tissue engineering, cancer treatment and the like.