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The application relates to the technical field of supramolecular, in particular to a ternary eutectic solvent as well as a preparation method and application thereof. The ternary eutectic solvent is prepared from component A, component B and component C, wherein the component A is a hydrogen bond donor, the component B is a hydrogen bond acceptor, and the component C is adenosine. The intermolecular interaction mode of the ternary eutectic solvent breaks the original strong force between adenosine molecules, so that the adenosine can be uniformly dispersed in the ternary DES system in the form of single molecules or small aggregates, thereby significantly improving the solubility of adenosine. Meanwhile, the existence of the ternary DES changes the dissolution and release behavior of the drug in the body, promotes the absorption of the drug, and greatly improves the bioavailability of adenosine.