The invention relates to a crystal form of warnemuline hydrochloride hydrate, a preparation method thereof and a pharmaceutical composition containing the crystal form. Specifically, the present invention relates to the crystalline form of warnimulin hydrochloride hydrate, the characteristic peaks 2θ of the crystalline form in the X-ray powder diffraction spectrum are at 9.2±0.2°, 9.5±0.2°, 10.3±0.2°, 11.8±0.2 °, 12.3±0.2°, 12.8±0.2°, 15.1±0.2°, 17.5±0.2°, 18.2±0.2° display. The warnemulin hydrochloride hydrate crystal form has a large bulk density, no hygroscopicity and good stability. The present invention also provides a method for preparing the above-mentioned warnemuline hydrochloride hydrate crystal form and a pharmaceutical composition containing the crystal form, which can be used in the preparation of various preparations, such as soluble powder, oral liquid, sustained release Granules, injections and other dosage forms have broad prospects for clinical use.