The invention discloses a process for jacking a continuous box girder incapable of releasing constraints at two ends at the same time. The process comprises the following steps: 1, releasing constraint at one end of the continuous box girder and setting a plurality of jacking points at the lower part of the continuous box girder; 2, arranging a jacking mechanism capable of performing jacking separately at each jacking point; 3, calculating and determining the jacking height of each jacking point, wherein the jacking heights H1, H2, H3, Hn-1 and Hn of the jacking points form a proportional linear relationship; 4, starting the jacking mechanisms, wherein the jacking points at the lower part of one end, with constraint, of the continuous box girder are not jacked and the other jacking points are correspondingly jacked to the heights H1, H2, H3, Hn-1 and Hn; 5, replacing or maintaining half or more of supporting seats close to one end, without constraint, of the continuous box girder and then resetting the jacking mechanisms and the continuous box girder; and 6, releasing constraint at the other end of the continuous box girder and repeating the steps. According to the process, the continuous box girder incapable of releasing the constraints at the two ends at the same time can be jacked and the supporting seats can be maintained or replaced, so that the safety of a continuous box girder structure is effectively protected.