The invention relates to a constant current source generation circuit, in particular to a constant current source generation circuit which is suitable for controlling current magnitude with direct current voltage, belonging to the technical field of an electronic circuit. The constant current source generation circuit comprises a sample amplifier U1, a summator U2, a triode N1, resistors R1, R2, R3, R4 and R5, a load and a power supply that are in circuit connection, wherein the sample amplifier U1 has sampling action for the power supply and is used for sampling the potential of the load, the summator U2 is an addition operation amplifier, the positive potential of the summator U2 is an intermediate value of the sample potential and the precise normal potential, and through two times of feedback amplification, the positive potential of the summator U2 is the sum of the sample potential and the precise normal potential; the output of the summator U2 is connected with a common base of the triode N1 to control the voltage output of the common base, the change the summator U2 is changed along with the change of the load sample, and then the voltage output is regulated by the triode N1 to reach potential balance. The constant current source generation circuit has the characteristics that the precision and the load power are improved, the circuit structure is simple, the cost is low, the control is easy, etc.