19 results about "Light microscopy technique" patented technology

The present invention provides a system and method of labeling a biological cell by at least one processor. Embodiments include obtaining a transmitted-light microscopy patch depicting the biological cell, extracting a context vector representing biological characteristics based on the microscopy patch, processing the patch through a machine-learning encoder to generate a feature vector representing the cell in a latent feature space, employing a machine-learning transformation model to augment the feature vector based on the context vector, and applying a generative decoder model on the augmented feature vector to predict at least one organelle image, wherein the predicted organelle image imitates an organelle-specific fluorescent image of the biological cell. Embodiments may further include presenting the predicted organelle image via a user interface, enabling a human expert to determine a state of the biological cell and / or biological sample based on the transmitted-light microscopy patch.

The invention relates to an apparatus for multimodal analysis of sample material, for example from a tissue, which detects molecular image information from the sample material in a spatially resolved manner, for example using a MALDI time-of-flight mass analyser, records light microscopy image information from the sample material in a spatially resolved manner, and associates the two with improved accuracy to give spatially resolved co-registered overall image information.

The application discloses a full-wavelength limit resolution structured light microscopic imaging method and related products, which can be applied to the technical field of optical microscopes. The method comprises the following steps: determining a target fringe period of a structured light illumination fringe based on an emission wavelength; adjusting the structured light illumination fringe to the target fringe period based on a spatial light modulator, so that a structured light microscopic imaging system reaches full-wavelength limit resolution; acquiring a first frequency domain image, a second frequency domain image and a third frequency domain image corresponding to a target sample based on the structured light illumination fringe; processing the first frequency domain image, the second frequency domain image and the third frequency domain image based on a frequency domain image multiplication algorithm, and determining a target wave vector; and performing image reconstruction on the target sample based on the target wave vector. In this way, the system maintains the limit resolution at any emission wavelength through the adjustment of the spatial light modulator, and then the frequency domain image multiplication algorithm accurately solves the target wave vector under the condition, thereby improving the accuracy of image reconstruction.

This invention relates to a method for controlling the quality of a composite structure comprising a thin film made of monocrystalline silicon carbide disposed on a carrier substrate made of polycrystalline silicon carbide, the method comprising: a) examining the free surface (10a) of the thin film (10) using a combination of confocal visible light microscopy and photoluminescence imaging, thereby enabling the detection of defects referred to as secondary defects; b) performing preliminary identification of secondary defects based on visible light images by means of similarity using an image recognition algorithm, the image recognition algorithm having been trained for various defect types such as holes, bubbles, scratches, and crystal source defects; after completing step b), each Secondary defects are all associated with an identified defect type and have a certain level of similarity; c) At least some secondary defects are finally classified by applying the following first set of conditions: - If the similarity level associated with a secondary defect is greater than a higher level, the secondary defect is finally classified as an identified defect type; - If the similarity level associated with a secondary defect is between a low level and a high level, the photoluminescence image of the defect is analyzed; if a secondary defect is associated with a labeled PL defect type, the secondary defect is finally classified as an identified defect type; - In other cases, the secondary defect is finally classified as a non-defect.

The invention relates to the technical field of optical imaging, and discloses a dark field polarized light microscopic imaging device, which comprises a dark field illumination light source; the light beam shaping system is arranged behind the light path of the dark field illumination light source; the polarizer is arranged behind the light path of the light beam shaping system; the dark field illumination ring is arranged behind the light path of the polarizer; the microscope objective is used for collecting signal light from the sample; the dark field imaging ring is arranged in an imaging light path of the microscope objective; the polarization analyzer is arranged behind the light path of the dark field imaging ring; and an imaging camera. The device provided by the invention has the advantage of high-sensitivity detection of a nano-scale scatterer by a dark-field microscope, and also obtains the capability of identifying and analyzing a material by a polarizing microscope by utilizing polarization characteristics, so that the signal-to-noise ratio and contrast ratio of imaging built-in nano impurities or defects in a strong birefringence material are remarkably improved; the problem of background interference which cannot be overcome by a single technical means is solved.

The present invention relates to the use of light microscopy for reticulocyte maturity classification of stained reticulocytes. In particular, the present invention relates to a method for reticulocyte maturity classification from a whole blood sample, comprising: staining the sample with a supravital agglutination stain reagent or a fluorescent agglutination dye; illuminating the stained sample with a light beam to detect reticulocytes; determining for each reticulocyte the parameters (i) the fraction of reticular area (Ar) relative to the whole cell area (Ac) (Λ) and (ii) the fraction of reticular perimeter (Ur) relative to the reticular area (Ar) (Γ); and classifying the reticulocyte maturity into one of four main maturity classes according to the determined Λ and Γ values.

Methods and systems for imaging a sample using fluorescence microscopy. High resolution lenses can be used for light sheet microscopy by tilting the excitation beam relative to the imaging optical axis. The excitation beam can be tilted using mirrors, instead of lenses, to generate the tilted illumination sheet. In some examples, a light path starts at a top downward-facing cone of light as it emerges from an optic fiber; a first off-axis parabolic mirror collimates in the x-axis, while a second off-axis parabolic mirror collimates in the y-axis, followed by the photomask to create four primary light sheets, which are then focused by a third off-axis parabolic mirror. As the four primary light sheets converge they create an interference pattern at an objective lens.

Provided herein is a method for preparing a castable flow cell body (120) of a flow cell unit (100), for imaging by light microscopy a sample under flow comprising at least one multicellular construct suspended in a sample carrier liquid, the method comprising: - adding a casting substance in a pourable state to a moulding cavity (222) of a mould assembly (200) configured for moulding the castable flow cell body (120) and a flow cell channel (140) in the castable flow cell body (120); - allowing or inducing the casting substance to reach a set state; and - releasing the castable flow cell body (120) so formed from the moulding cavity (222); wherein the castable flow cell body (120) in the set state has a refractive index matching a refractive index of the sample carrier liquid.

This invention discloses a method for constructing a mouse model of COL4A5-K229X point mutation X-linked Alport syndrome. Cas9 mRNA, gRNA, and a donor vector were designed and prepared targeting the c.685A>T point mutation in exon 12 of the mouse COL4A5 gene. These components were mixed and microinjected into C57BL / 6J mouse zygotes to obtain F0 generation mice. Genotypes were identified by PCR amplification and Sanger sequencing, and positive mice were screened. Positive F0 generation mice were mated with wild-type mice to breed F1 and subsequent generations. The model phenotype was further verified by qPCR, biochemical analysis, light microscopy, transmission electron microscopy, and immunofluorescence. The model constructed by this invention exhibits hematuria, proteinuria, azotemia, podocyte loss, and irregular thickening and stratification of the glomerular basement membrane, consistent with the phenotype of human XLAS patients, providing an animal model tool for elucidating the pathogenesis and developing treatment strategies.

Disclosed are probes that are expressed in a cell to label an intracellular target (such as protein or DNA) for both light and electron microscopy. The probes comprise a targeting domain that specifically binds to the intracellular target, a detection tag that can be used to detect the intracellular location of the probe using light microscopy, and a ferritin nanoparticle ferritin nanoparticle with ferroxidase activity and that stores ferric oxide. Also disclosed are nucleic acids encoding the fusion proteins and methods of their use.

A method for the spatiotemporal mapping of receptor-ligand binding kinetics in localized surface plasmon resonance (LSPR) imaging using a chip for LSPR imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array. The nanostructures can be regenerated allowing the chip to be used multiple times.

The utility model relates to the technical field of portable optical microscopes, and discloses a portable optical microscope which comprises a supporting plate, a supporting frame is fixedly installed on the upper surface of the supporting plate, and a microscope body is movably installed on the side wall of the supporting frame. According to the portable optical microscope, the microscope body can move on the supporting plate, the carrying size is reduced, carrying is convenient, when the portable optical microscope needs to be used, the supporting plate is placed on the ground, a knob is adjusted to enable a threaded rod to move, the supporting plate is driven to move, and flatness of the microscope body is guaranteed; an extrusion rod penetrates through an internal through groove of a threaded rod and penetrates out of a movable hole in a supporting plate to be inserted into the ground, along with the penetration of the extrusion rod, a rubber ring makes contact with a fixing groove to keep the insertion stability of the extrusion rod, infirm fixation is prevented, and the microscope body is turned over, so that the microscope is convenient to carry and use outdoors.

The invention discloses an isosteviol crystal form I as well as a preparation method and application thereof, the isosteviol crystal with the crystal form I has obvious characteristic diffraction peaks at least at positions of 9.98, 13.54, 15.88, 16.60, 17.26, 18.56, 19.30, 20.76 and 26.04 of 2 theta angles (+ / -0.02) expressed by degrees through X-ray powder diffraction analysis measured by a Cu-K alpha ray, and the isosteviol crystal with the crystal form I has the characteristic diffraction peaks at least at positions of 9.98, 13.54, 15.88, 16.60, 17.26, 18.56, 19.30, 20.76 and 26.04 of the 2 theta angles (+ / -0.02) expressed by degrees. The crystal is characterized by chemical analysis means such as X-ray powder diffraction analysis (XRD), thermogravimetric analysis (TG), a polarizing microscope and infrared spectroscopy (IR), the crystal has the advantages of high crystallinity, high stability, high biological accessibility and the like, the crystal structure of the crystal is still kept stable after the crystal is placed at normal temperature for 12 months, and the crystal has a good application prospect. The method is suitable for wider application fields.

The invention relates to an illumination and imaging method for light microscopy, wherein the illumination occurs at different axial positions in a sample or parts of the sample are imaged from different depths. The invention further relates to localization methods for individual emitters in a sample using such an illumination method, in particular to localization methods based on the MINFLUX principle. Finally, the invention comprises a light microscope for implementing an illumination, imaging, and / or localization method according to the invention.

The invention discloses a full-wavelength limit resolution structured light microscopic imaging method and related products, which can be applied to the technical field of optical microscopes, and the method comprises the following steps: determining a target fringe period of a structured light illumination fringe based on the wavelength of emitted light; the structured light illumination stripes are adjusted to a target stripe period based on a spatial light modulator, so that the structured light microscopic imaging system reaches the full-wavelength limit resolution; based on the structured light illumination stripes, obtaining a first frequency domain image, a second frequency domain image and a third frequency domain image corresponding to the target sample; processing the first frequency domain image, the second frequency domain image and the third frequency domain image based on a frequency domain image multiplication algorithm, and determining a target wave vector; and performing image reconstruction on the target sample based on the target wave vector. Thus, through adjustment of the spatial light modulator, the system can maintain the limit resolution under any emission light wavelength, and then a frequency domain image multiplication algorithm is provided to accurately solve the target wave vector under the condition, and the accuracy of image reconstruction is improved.

Systems, methods, and devices for providing Köhler illumination for inspection, under a microscope, of end connectors of fiber optic cables. In one aspect, a microscopy system provides Köhler illumination where a numerical aperture of the illumination (NAIL) is greater than a numerical aperture of imaging (NAIM). In another aspect, a reflected light microscopy system includes an array of light sources which illuminate a diffuser, which, in turn, projects the diffused light to reflect off of a beamsplitter and through an objective / condenser upon the end connector. In yet another aspect, the array of light sources may provide different light patterns to illuminate the end connector, and each light pattern may correspond to a different type of radiused end connector.

A microscopy imaging system comprises a fluorescence lifetime imaging microscopy (FLIM) system comprising a pulsed light source configured to direct a plurality of excitation light pulses onto a sample, a photo detector configured to detect emitted fluorescent photons created by the plurality of excitation pulses interacting with the sample, and a FLIM data acquisition system configured to measure the time interval between the excitation light pulses and the detected emitted fluorescent photons, a scanning light microscopy (SLM) system comprising a SLM data acquisition system, a fast scanning mirror and a slow scanning mirror, wherein the mirrors are configured to scan the light pulses across the sample; and a data processing system communicatively connected to the FLIM and SLM systems. Microscopy imaging methods are also disclosed.

The invention relates to a light microscopy method, wherein a sample (2) is illuminated with focused pulsed excitation light (A) to excite at least two types of emitters (E) with different emission lifetimes to emit light, wherein the sample (2) is additionally illuminated with an intensity distribution of suppression light (V) with a local minimum to de-excite emitters (E) outside the local minimum, wherein photons emitted from the sample (2) are detected by a detector (4), wherein a phasor analysis is performed on the basis of the detected photons (P) to separate photons of the different types of emitters (E) based on their emission lifetime, wherein photon gating is performed, wherein for excitation pulses (p A) of the excitation light (A) respective gating time intervals (10) are provided, and wherein in the phasor analysis only those photons (P) are taken into account which are detected in the respective gating time interval (10), as well as a light microscopic device (1) and a computer program for carrying out the procedure.

The invention relates to the technical field of optical microscopes, and discloses an extensible multifunctional inverted microscopic spectrum and imaging system, which comprises an integrated three-layer machine body frame, on the top-layer optical bread board, the objective lens and a three-axis displacement table for bearing a sample are rigidly fixed on the same platform; an electric sliding table type switching module and a magnetic quick-release optical filter assembly are arranged on the bottom layer optical bread board side by side. The electric sliding table module is used for quickly switching between a microscopic imaging light path and a spectral analysis light path; the magnetic quick-release optical filter assembly is used for quickly replacing optical sets with different excitation wavelengths. The system stability is fundamentally guaranteed through the common platform design, seamless and efficient switching of multi-mode measurement is achieved through a dual-module collaborative switching mechanism, meanwhile, the system is endowed with excellent flexibility and future expansibility through the open structure, and a comprehensive platform is provided for scientific research.