The disclosed technology brings
histopathology into the operating theatre, to enable real-time intra-operative
digital pathology. The disclosed technology utilizes
confocal imaging devices image, in the operating theatre, “optical slices” of
fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen
section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital
histology scanner, with the capability of rapidly scanning all outer margins of a
tissue sample (e.g., resection lump, removed tissue
mass). Using point-
scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the
digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the
tissue sample itself.