The invention discloses a non-contact intraocular pressure detection system based on low-coherence optical interference. The system comprises an optical division and return optical path, a signal acquisition and calibration optical path, a reference arm optical path, a data acquisition and storage control module, a terminal data processing module and a display and mechanical control terminal. According to the system, intraocular pressure is measured on the premise that the inner surface layer of the cornea is pressed flat, a longitudinal three-dimensional method is adopted to process data, a fixation light source is arranged at the end, coaxial with a first convergent lens, of a semi-reflecting and semi-transmitting lens in the signal acquisition and calibration optical path, so that a scanning light source and the fixation light source are integrated to be coaxial with the gas injection direction, and therefore intraocular pressure measurement precision and speed are increased; besides, a light beam needs to be subjected to luminous power detection before entering the eyeball, so that detection safety is improved; meanwhile, in a gas generation device providing jet streams, a purifier is arranged between a piston and an air cavity, so that cleanness of jet streams is guaranteed; and due to the fact that the whole system is debugged before normal operation, safety, reliability and stability of the whole measurement process are guaranteed.