The invention belongs to the technical field of microneedles, and particularly discloses a microneedle female die, a female die, a preparation process of the female die, a microneedle patch and a preparation process of the microneedle patch. The microneedle female die comprises a PDMS forming groove and a defoaming film, and a plurality of microneedle protrusions are arranged in the PDMS forming groove in an array mode. And the defoaming film is attached to the upper part of the PDMS forming groove. When the female die is prepared from the female die, interference PDMS mixed liquid is added into the PDMS forming groove, the bubble removing film is attached to the upper portion of the PDMS forming groove to seal the PDMS mixed liquid in the PDMS forming groove, and bubble-free planar shaping treatment is conducted on the female die. The female die obtained by recarving the female die is good in integrity, and the polymer microneedle in any form can be prepared; the microneedle produced through the female die has the advantages of being accurate in drug delivery, controllable, stable, easy to produce, capable of saving materials and capable of being flexibly transformed, the yield and the preparation efficiency can be effectively improved, and the cost is reduced.