The invention relates to a laser cladding bonding-based cutter and workpiece diffusion couple making method. The method mainly comprises the following steps: 1, cutting a cutter or blade made of a known material to form a sample, and allowing the cut sample to have at least one groove or square cavity; 2, cutting a workpiece to be tested to form a sample, and laminating the cut sample to the sample obtained in step 1 to realize at least one common groove or square cavity; 3, grinding and polishing obtained two samples to make the two samples respectively have at least one polished surface; 4, laminating the polished surfaces of the two samples, clamping and fixing the two samples by using a clamp, and placing a cladding material in the groove or square cavity formed by the two samples; 5, bonding the two samples through a laser cladding technology to form a diffusion couple; 6, cleaning the produced diffusion couple, and carrying out heating and heat insulation in a heat insulation furnace; and 7, taking out the heat insulated diffusion couple, taking out a surface oxidation layer, polishing and cleaning, taking the core of the diffusion couple, and carrying out sample analysis.