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The invention discloses a qualitative and quantitative determination method of lactobacillus plantarum intestinal tract colonization. The qualitative determination method comprises the following steps of (1) conducting total deoxyribonucleic acid (DNA) extraction on excrement samples to be measured and pretreated; (2) conducting polymerase chain reaction (PCR) amplification of total DNA; (3) conducting PCR amplification of a V3 area; (4) conducting denaturing gel gradient electrophoresis on products after the PCR amplification of the V3 area and conducting gel imaging gel analysis; and (5) enabling a stripe of denaturing gradient gel obtained in the step (4) to be compared with a blank group and a comparing marking group. The quantitative determination method comprises the following steps of operating samples to be measured and lactobacillus plantarum series concentration bacterium powder according to the step (1) to the step (4), then building a standard curve for luminance of a stripe on series concentration lactobacillus plantarum denaturing gradient gel and contents, and finally calculating colonization lactobacillus plantarum quantity according to the standard curve.

Disclosed is a pharmaceutical composition of red yeast and ginkgo leaves for preventing and treating cardiovascular and cerebrovascular diseases, and the use of the composition in preparing health products or food additives. The composition is prepared from red yeast and ginkgo leaves by the weight ratio of 1-5:5-1 as active constituents.

Disclosed is a semiconductor avalanche tolerance failure analyzing and testing method and device based on a thermal imaging technology. The semiconductor avalanche tolerance failure analyzing and testing method based on the thermal imaging technology mainly includes that, in a constructed test system, a to-be-tested power device and a power switch tube are turned on, an inductor starts to stream,the to-be-tested power device and the power switch tube are turned off after a current in the inductor reaches an avalanche current peak value Iav, the inductor discharges, and avalanche occurs in theto-be-tested power device, a short-circuit power device is turned on before the to-be-tested power device is broken through, the to-be-tested power device is subjected to a short circuit and the avalanche stops, energy of the inductor is discharged by the short-circuit power device, the operation is repeated afterwards, so that avalanche occurs continuously in the to-be-tested power device, the avalanche time of the device is limited so that an avalanche process is performed repeatedly without damaging the device, and in this process, changes of the locations of heating points during the avalanche process can be observed by the thermal imaging technology.

The invention provides a skin safety evaluation method on nanometer titanium dioxide based on HaCaT cells, and belongs to the field of external material detection. The method comprises the following steps that the HaCaT cells are sequentially treated by nanometer titanium dioxide solutions with different concentrations and ultraviolet rays, and a cell survival rate is tested; the concentration ofthe nanometer titanium dioxide solution with the HaCaT cell survival rate reaching 50 to 90 percent is selected to be used as potential dangerous concentration; the ultraviolet ray treatment is combined, so that a sialic acid expression level in the cells and an ROS level in the cells generate constant change; finally, the influence of the potential dangerous concentration of nanometer titanium dioxide on the cell injury degree can be judged through detecting the change of the expression level of the sialic acid, so that a set of standard nanometer titanium dioxide skin safety evaluation method is obtained. The invention provides the reliable scientific basis for the safe use of the nanometer titanium dioxide and also provides reference for the safety evaluation of other nanometer materials.

The invention discloses a bionic tadpole device based on IPMC driving and a driving method thereof. The bionic tadpole device is characterized in that a storing plate used for storing an IPMC driver is arranged on the maximum section of a cavity inside a head shell, a battery pack is stored in a space below the storing plate, and a button switch closely attached to the lower portion of the batterypack is arranged. A switch through hole is formed in the position, connected with the shell, of the bottom of the battery pack, and the button switch stretches outwards from the switch through hole.The junction of an IPMC driving material and a tail is connected and sealed by silica gel. The IPMC driver and the IPMC driving material at the tail are connected at one end in the shell through an externally-connected electrode sheet. According to the deformation characteristics of the IPMC material, an intelligent IPMC material is adopted as the driving material; the controller adopts a controldevice mainly provided with a single chip microcomputer, and the frequency and voltage can be adjusted; and the bionic tadpole device is simple in structure, convenient in power supplying and small insize, the research of bionics is extended, and meanwhile, deeper research and promotion are made for adopting the IPMC material as the driving material of a bionic robot.

The invention discloses a method for adjusting mitochondrial gene expression by using small RNA and application thereof. Adjustment of the mitochondrial gene expression can be realized by transfecting siRNA or miRNA into cells. Through the method, the functions of a mitochondrial gene and a mitochondrion adjusting function can be researched. By transferring the siRNA of a mitochondrial target gene into cells, the abundance degree of the mRNA of the target gene in the mitochondrion can be reduced and the protein level expression is reduced. The expression and translation levels of the target gene can be enhanced by the miRNA under the condition of not changing the mRNA level of the target gene in the mitochondrion and the function of the mitochondrion is adjusted. According to the invention, an adjusting effect of the miRNA on the mitochondrial gene is found firstly and a new direction is provided for research on the functions of the mitochondrial gene.

The invention discloses a method for evaluating a schottky current transportation mode of a semiconductor device, and belongs to the technical field of methods for evaluating characteristic physical mechanisms of semiconductor devices. The method comprises the following steps of: acquiring logI-V relation curves of the device under different temperature conditions, fitting and analyzing slopes of the acquired logI-V relation curves, and comparing the slope values of the logI-V relation curves under different temperature conditions to evaluate the schottky current transportation mode of the semiconductor device. By the method for evaluating the schottky current transportation mode of the semiconductor device, a current transportation mechanism of the semiconductor device can be determined, so that a potential barrier height and a Richardson constant of the semiconductor device can be accurately acquired; and therefore, the physical mechanism of the semiconductor device can be deeply researched.

The invention provides an ENO2 monoclonal antibody 1C5 as well as a preparation method and application thereof, and relates to the technical field of monoclonal antibodies. The invention discloses a complementary determining region sequence of variable regions of a heavy chain and a light chain of the monoclonal antibody 1C5, the variable region sequence can be constructed on an antibody expression vector based on the sequence, and a recombinant monoclonal antibody is obtained by transfecting cell expression. The monoclonal antibody 1C5 vector obtained by the invention is easy to store, and the quality control in the antibody production process is easy; the monoclonal antibody 1C5 disclosed by the invention can recognize the ENO2 protein, has biological activity, can be used for ENO2 antigen detection and other scientific researches, and has very good application value and very important scientific research guiding significance.

The invention provides an intracellular and external electrophysiological recording system and method for automatic electroporation regulation and screening. The system comprises a microelectrode array sensor, an intracellular and external electrical signal conditioning module, an electroporation control module, a data acquisition card, an upper computer and a power supply module. By means of the system, the functions of cell electroporation and myocardial cell intracellular and extracellular electric signal recording can be achieved on one instrument, and the channels can be controlled to generate pulse signals with different frequencies, amplitudes and pulse widths one by one and apply the pulse signals to cells. Therefore, comparison of electroporation results under different conditions in one experiment can be realized, change of cell electrophysiological phenomena can be detected, and cell electric signal recording with high temporal-spatial resolution is realized. Meanwhile, efficient and automatic analysis can be realized through the upper computer, the optimal condition of electroporation is screened out by traversing different electroporation conditions and combining data analysis, and cell electroporation is performed under the optimal condition, so that intracellular electric signals of myocardial cells can be recorded in a high-quality manner.

The invention relates to an optical system for a microcell spatial coherent pattern and an operating method thereof. The system comprises a microscope (2), a first total reflective mirror (3), a first lens (4), a double-slit unit (5), a first double-slit interference imaging lens (7), and a second total reflective mirror (9) that are arranged successively along an optical path. In addition, the system also includes an imaging lens (11), a third total reflective mirror (13), a second double-slit interference imaging lens (14), and a charge coupling device (16) that are arranged successively along the reflective optical path of the second total reflective mirror (9). The double-slit unit, the first double-slit interference imaging lens, the second total reflective mirror, the imaging lens, and the second double-slit interference imaging lens are movable elements. Different optical paths are formed by arranging or removing all movable elements at the optical path. In addition, the invention also provides an operating method of the system. With the method and system, with collection of the interference pattern, the spatial coherence and the coherent area of the light source under the micro-nano scale are measured, so that defects of the existing optical microscope system are overcome.

The invention provides a device and a method for testing wet-state springback capability of an AGM partition plate under different compression amounts, and belongs to the technical field of storage batteries. The device comprises a main rack, a clamping assembly, a lifting assembly, a data reading assembly and a liquid dropping assembly, wherein the main rack comprises a bottom support and a vertical beam arranged on the bottom support, the clamping assembly comprises a cushion block arranged on the upper end surface of the bottom support and a cover sheet arranged on the upper end surface of the cushion block, the lifting assembly is arranged on the vertical beam, a pressure applying end is connected to the lifting assembly, the data reading assembly comprises a pressure reading component and a displacement reading component, the pressure reading component is arranged on the lifting assembly, the displacement reading component is arranged on the vertical beam and connected with the lifting assembly, the liquid dropping assembly comprises a liquid dropping bracket arranged on one side of the main rack and a liquid dropping pipe longitudinally arranged on the liquid dropping bracket, and the liquid dropping pipe is connected with a flow guide nozzle through a flow guide pipe. By means of the device for testing the wet-state springback capability of the AGM partition plate under the different compression amounts, the AGM partition plate can be known and researched more deeply.

The invention belongs to the field of medicines, and relates to a 1, 2, 4-triazole derivative compound and application thereof in treatment of inflammatory bowel diseases. The structural formula of the 1, 2, 4-triazole derivative compound is shown as a formula I. In a 3% dextran sodium sulfate induced mouse ulcerative colitis model, the compound prepared by the invention has a remarkable improvement effect.