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The invention relates to the technical field of genetic engineering, in particular to a double sgRNA targeted to knock out the FRZB gene and a porcine fibroblast cell line for knocking out the FRZB gene and applications thereof. The present invention provides a double sgRNA targeted to knock out the FRZB gene, which cuts two sites on the first exon of the FRZB gene, and then constructs the sgRNA into an expression vector and then transfects pig fibroblasts through an optimized electroporation system cells, and then use flow cytometry to sort out high-purity positive monoclonal cells, and obtain FRZB knockout cell lines through genomic identification and screening. The invention solves the problems of low transfection efficiency, low targeting efficiency and low monoclonal purity in the process of constructing a knockout cell line, and is easy to operate and obtains an edited cell line with longer segment deletions. The involved cell lines can be used as donors of somatic cell nuclear transfer for the preparation of transgenic pigs, which provide a better research tool for deeply exploring the biological functions of FRZB genes.

The invention discloses a social network data privacy protection method. The condition that an original social network graph is possibly secure during release is considered, but the privacy leakage problem possibly exists for each sub-graph after community detection; a community structure is stored as far as possible under the condition of ensuring the security of each sub-graph; and privacy leakage in communities, privacy leakage between the communities, and different measures for the two leakage modes are proposed. According to the method, structure characteristics of the graph are ensured under the condition of ensuring user privacy security of each sub-community, so that the divided communities can be subjected to further research better and more detailedly.

The invention discloses a method and application of small RNA regulating mitochondrial gene expression. Regulation of mitochondrial gene expression can be achieved by transfecting siRNA or miRNA into cells. This method can be used to study the function of mitochondrial genes and regulate mitochondrial function. Transferring siRNA of mitochondrial target genes into cells can effectively reduce the abundance of target gene mRNA in mitochondria, and also reduce the expression of protein level. miRNA can enhance the expression and translation level of target genes and regulate the function of mitochondria without changing the mRNA level of target genes in mitochondria. The invention discovers for the first time the regulating effect of small RNA on mitochondrial genes, and provides a new direction for studying the functions of mitochondrial genes.

The invention discloses a rotary-jet-grouting solidifying hole sealing simulation test platform and a test method. The test platform is mainly composed of a coal / rock mass fixing table, a rotary-jet-grouting drilling machine, a high-pressure grouting pump, a slurry stirring device, a sealing-effect inspecting device and the like. The test method includes the steps that screened coal / rock powder, water and other preparing materials are mixed to be even to be poured into the coal / rock mass fixing table; after a test block is solidified and molded, a drill hole is constructed in the test block through the rotary-jet-grouting drilling machine; in the process of drawing back a drilling rod, sealing slurry is subjected to rotary jet grouting while lifting the drilling rod, the sealing slurry is evenly sprayed to the inner wall of the drill hole and forms a sealing layer, the slurry permeates into fractures / gaps in the periphery and is solidified, and communication between the drill hole and the fractures / gaps in the periphery is isolated. In order to obtain the optimal sealing effect, the parameters of the devices and the ratio of the slurry need to be adjusted, and testing is repeated. The effect is tested through the specially-made inspecting device, and the tested result is of important practical significance in selection of the lifting speed, the rotating speed, the rotary-jet-grouting pressure, the rotary-jet-grouting flow and the ratio of the slurry.

A drift time ion migration spectral device comprises a drift tube. The drift tube comprises the components of a first ion gate, a second ion gate and an outlet mesh. Through controlling the state of the second ion gate, the state of sample ions after electromigration of a front drift region between the first ion gate and the second ion gate is controlled. When the state of the second ion gate is open, the sample ions after electromigration enter a back drift region between the second ion gate and the outlet mesh through the second ion gate, and furthermore arrive at a detector through the outlet mesh. According to the drift time ion migration spectral device, through arranging the double ion gates, the drift time ion migration spectral device has advantages of simplifying spectrum, enlarging signal-to-noise ratio of the spectrum and eliminating interference of reactive ions, realizing convenient switching among various functions, and improving deeper research on a substance.