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The invention discloses a polymorphic sampling system and method for sulfur trioxide in exhaust of a stationary pollution source. The polymorphic sampling method comprises the following steps: according to the form of SO3 in the exhaust, based on a selective condensation principle, collecting aerosol-state SO3 in the exhaust by adopting a front-stage collector under the temperature of the exhaust; and enabling the temperature of the exhaust flowing in a rear-stage collector to be reduced below an acid dew point through a water bath or water bath circulation so that the original gaseous SO3 under the temperature of the exhaust is transformed into the aerosol-state SO3 to be deposited in the rear-stage collector so as to realize the purpose that the aerosol-state SO3 and the gaseous SO3 are respectively measured. According to the scheme provided by the invention, the aerosol-state SO3 and the gaseous SO3 in the exhaust can be distinguished, and respectively quantified; a proportion of the aerosol-state SO3 to the gaseous SO3 is calculated, and the total concentration of the SO3 can also be measured. The measured concentration of the aerosol-state SO3 and the gaseous SO3 under the temperature of the exhaust provides basic data for researching acid corrosion at the tail of a boiler; meanwhile, the polymorphic sampling system can be applied to detection of SO3 emission concentration of exhaust of other pollution sources.

The invention relates to the technical field of genetic engineering, in particular to a double sgRNA targeted to knock out the FRZB gene and a porcine fibroblast cell line for knocking out the FRZB gene and applications thereof. The present invention provides a double sgRNA targeted to knock out the FRZB gene, which cuts two sites on the first exon of the FRZB gene, and then constructs the sgRNA into an expression vector and then transfects pig fibroblasts through an optimized electroporation system cells, and then use flow cytometry to sort out high-purity positive monoclonal cells, and obtain FRZB knockout cell lines through genomic identification and screening. The invention solves the problems of low transfection efficiency, low targeting efficiency and low monoclonal purity in the process of constructing a knockout cell line, and is easy to operate and obtains an edited cell line with longer segment deletions. The involved cell lines can be used as donors of somatic cell nuclear transfer for the preparation of transgenic pigs, which provide a better research tool for deeply exploring the biological functions of FRZB genes.

The invention discloses a social network data privacy protection method. The condition that an original social network graph is possibly secure during release is considered, but the privacy leakage problem possibly exists for each sub-graph after community detection; a community structure is stored as far as possible under the condition of ensuring the security of each sub-graph; and privacy leakage in communities, privacy leakage between the communities, and different measures for the two leakage modes are proposed. According to the method, structure characteristics of the graph are ensured under the condition of ensuring user privacy security of each sub-community, so that the divided communities can be subjected to further research better and more detailedly.

The invention discloses a method and application of small RNA regulating mitochondrial gene expression. Regulation of mitochondrial gene expression can be achieved by transfecting siRNA or miRNA into cells. This method can be used to study the function of mitochondrial genes and regulate mitochondrial function. Transferring siRNA of mitochondrial target genes into cells can effectively reduce the abundance of target gene mRNA in mitochondria, and also reduce the expression of protein level. miRNA can enhance the expression and translation level of target genes and regulate the function of mitochondria without changing the mRNA level of target genes in mitochondria. The invention discovers for the first time the regulating effect of small RNA on mitochondrial genes, and provides a new direction for studying the functions of mitochondrial genes.

The invention discloses a rotary-jet-grouting solidifying hole sealing simulation test platform and a test method. The test platform is mainly composed of a coal / rock mass fixing table, a rotary-jet-grouting drilling machine, a high-pressure grouting pump, a slurry stirring device, a sealing-effect inspecting device and the like. The test method includes the steps that screened coal / rock powder, water and other preparing materials are mixed to be even to be poured into the coal / rock mass fixing table; after a test block is solidified and molded, a drill hole is constructed in the test block through the rotary-jet-grouting drilling machine; in the process of drawing back a drilling rod, sealing slurry is subjected to rotary jet grouting while lifting the drilling rod, the sealing slurry is evenly sprayed to the inner wall of the drill hole and forms a sealing layer, the slurry permeates into fractures / gaps in the periphery and is solidified, and communication between the drill hole and the fractures / gaps in the periphery is isolated. In order to obtain the optimal sealing effect, the parameters of the devices and the ratio of the slurry need to be adjusted, and testing is repeated. The effect is tested through the specially-made inspecting device, and the tested result is of important practical significance in selection of the lifting speed, the rotating speed, the rotary-jet-grouting pressure, the rotary-jet-grouting flow and the ratio of the slurry.

A drift time ion migration spectral device comprises a drift tube. The drift tube comprises the components of a first ion gate, a second ion gate and an outlet mesh. Through controlling the state of the second ion gate, the state of sample ions after electromigration of a front drift region between the first ion gate and the second ion gate is controlled. When the state of the second ion gate is open, the sample ions after electromigration enter a back drift region between the second ion gate and the outlet mesh through the second ion gate, and furthermore arrive at a detector through the outlet mesh. According to the drift time ion migration spectral device, through arranging the double ion gates, the drift time ion migration spectral device has advantages of simplifying spectrum, enlarging signal-to-noise ratio of the spectrum and eliminating interference of reactive ions, realizing convenient switching among various functions, and improving deeper research on a substance.

Disclosed is a semiconductor avalanche tolerance failure analyzing and testing method and device based on a thermal imaging technology. The semiconductor avalanche tolerance failure analyzing and testing method based on the thermal imaging technology mainly includes that, in a constructed test system, a to-be-tested power device and a power switch tube are turned on, an inductor starts to stream,the to-be-tested power device and the power switch tube are turned off after a current in the inductor reaches an avalanche current peak value Iav, the inductor discharges, and avalanche occurs in theto-be-tested power device, a short-circuit power device is turned on before the to-be-tested power device is broken through, the to-be-tested power device is subjected to a short circuit and the avalanche stops, energy of the inductor is discharged by the short-circuit power device, the operation is repeated afterwards, so that avalanche occurs continuously in the to-be-tested power device, the avalanche time of the device is limited so that an avalanche process is performed repeatedly without damaging the device, and in this process, changes of the locations of heating points during the avalanche process can be observed by the thermal imaging technology.

The invention discloses a device for trapping smoke of a heat-not-burn cigarette. The device is characterized by mainly comprising a worktable (14), a gas generator (1), an electric heating device and a smoke trapping device, wherein the gas generator (1), the electric heating device and the smoke trapping device are arranged on the worktable (14); the electric heating device is used for heating the cigarette (3), the gas generator (1) is used for providing a low-oxygen gas environment for heating the cigarette (3), and the smoke trapping device is used for collecting the smoke produced by the heated cigarette (3). The device has the advantages that not only is the structure simple, but also the cost is low; the release amount of harmful ingredients of the novel heat-not-burn cigarette can be obtained by carrying out qualitative and quantitative analysis on the smoke of the cigarette, and an analysis result can be used for evaluating various indexes of the novel heat-not-burn cigarette so as to carry out in-depth study on the novel cigarette, so that the device is suitable for generalization and application.