The invention discloses a sterilization treatment device for a urine sample in a clinical laboratory. The sterilization treatment device mainly comprises a liquid inlet funnel, an inner shell and an outer shell, wherein the liquid inlet funnel comprises a liquid inlet, a draught fan, a filter net, a deodorizer and a flow valve; the inner shell comprises a liquid inlet pipeline, an autoclaved sterilization boiler, an ozone generator, an ozone disinfector, an exhaust pipe, a pressurizing container, a volume sensor, a power supply, an external power supply plug, a waste liquid storage container, a liquid storage pipeline, an ozone pipeline and an electric heating wire; and the outer shell comprises an exhaust hole, an ozone disinfection spray head, an alarm and a waste liquid draining hole. The sterilization treatment device is small and exquisite and is flexible to move; and after various types of urine samples are collected, the urine samples are filtered, pressured and subjected to autoclaved sterilization and ozone disinfection, so that the safety emission is realized, and no any environmental pollution is caused.