The invention mainly relates to a bottom valve and piston valve assembly line, comprising a PLC (Programmable Logic Controller) control cabinet, a rack, a shadowless lamp frame, a dustproof cover, two columns of conveying lines which have opposite transmission directions and are mounted on the rack in parallel, a tool board, a transferring device for transferring the tool board, and a driving part. The transferring device is provided with two groups of double-ended heads which are respectively mounted on the conveying lines; the tool board is located on the conveying line; the dustproof cover and the shadowless lamp frame are mounted on the rack; the shadowless lamp frame is higher than the dustproof cover; and a shadowless lamp is mounted at the top of the shadowless lamp frame. The bottom valve and piston valve assembly line provided by the invention has the advantages of beautiful and elegant appearance, small equipment size, and small occupied area, and can be used for effectively saving space and field for a user. With the adoption of the assembly line, the automatic conveying is realized, so that the labor productivity is greatly improved, and the labor is saved. Meanwhile, a photoelectric error-prevention system is arranged, so that the problem of assembly error caused by personal factors can be effectively solved, and the product quality is greatly improved.